Defined serum-free media for in vitro expansion of adipose-derived mesenchymal stem cells
Defined serum-free media for in vitro expansion of adipose-derived mesenchymal stem cells
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DOI：
10.1016/j.jcyt.2014.02.006
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发表时间：
2014-07-01
期刊：
影响因子：
4.5
通讯作者：
Gotherstrom, Cecilia
中科院分区：
文献类型：
--
作者：
Al-Saqi, Shahla Hamza;Saliem, Mohammed;Gotherstrom, Cecilia
背景。人们对间充质干细胞（MSC）越来越感兴趣，因为它们被认为是细胞治疗的良好候选者。脂肪组织是一种易于获取的来源，可以非侵入性地大量获取间充质干细胞（Ad-MSC）。本研究的目的是评估用于体外扩增 MSC 的确定的无血清培养基，作为其临床使用的先决条件。方法。从健康捐赠者身上分离出脂肪组织。分离细胞并在无血清培养基 (Mesencult-XF) 和补充有 10% 胎牛血清的 Dulbecco 改良 Eagle 培养基 (DMEM-FBS) 中扩增 5 代。评估了 MSC 形态、标志物表达、活力、群体倍增时间以及向成骨和脂肪形成谱系的分化潜力。骨髓 MSC 作为对照。结果。与在 DMEM-FBS 中培养的 Ad-MSC 相比，在 Mesencult-XF 中培养的 Ad-MSC 具有更短的群体倍增时间（33.3 +/- 13.7 h）（54.3 +/- 41.0 h，P < 0.05）。与在 DMEM-FBS 中培养的 Ad-MSC 相比，在 Mesencult-XF 中培养的 Ad-MSC 表现出稳定的形态和表面标记表达以及更高的分化潜力。结论。确定的无血清和无异种物质的 Mesenult-XF 培养基似乎是 Ad-MSC 的不错选择，但当细胞用于临床目的时，它在支持骨髓 MSC 培养方面效果不佳。
Background. There is a growing interest in mesenchymal stem cells (MSCs) because they are regarded as good candidates for cell therapy. Adipose tissue represents an easily accessible source to derive mesenchymal stem cells (Ad-MSCs) non-invasively in large numbers. The aim of this study was to evaluate a defined serum-free medium for in vitro expansion of MSCs as a prerequisite for their clinical use. Methods. Adipose tissue was isolated from healthy donors. Cells were isolated and expanded for five passages in serum-free medium (Mesencult-XF) and Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum (DMEM-FBS). MSC morphology, marker expression, viability, population doubling time and differentiation potential toward osteogenic and adipogenic lineages were evaluated. Bone marrow MSCs were included as controls. Results. Ad-MSCs cultured in Mesencult-XF had shorter population doubling time (33.3 +/- 13.7 h) compared with those cultured in DMEM-FBS (54.3 +/- 41.0 h, P < 0.05). Ad-MSCs cultured in Mesencult-XF displayed a stable morphology and surface marker expression and a higher differentiation potential in comparison to Ad-MSCs cultured in DMEM-FBS. Conclusions. The defined serum-free and xeno-free Mesencult-XF media appear to be a good choice for Ad-MSCs, but it is not as good in supporting culture of bone marrow MSCs when the cells are to be used for clinical purposes.