Neuroendovascular optical coherence tomography imaging and histological analysis.
Neuroendovascular optical coherence tomography imaging and histological analysis.
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DOI：
10.1227/neu.0b013e318212bcb4
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发表时间：
2011-08
期刊：
影响因子：
4.8
通讯作者：
Chen Z
中科院分区：
文献类型：
--
作者：
Mathews MS;Su J;Heidari E;Levy EI;Linskey ME;Chen Z
血管内光学相干断层扫描（OCT）是最近发展起来的一种光学成像技术，它基于组织对近红外光或红外光的反射，提供来自完整组织的高分辨率横截面原位图像。报告神经血管内OCT成像的可行性，并在动物、尸体和临床研究中比较神经血管内OCT结果与非病变血管的组织学。在2头猪的颈总动脉和3例患者的颅内颈动脉中进行了基于导管的体内血管内OCT成像。通过腹股沟入路并使用标准血管内手术将血管内OCT器械输送到预期位置。使用外部马达通过旋转和平移扫描获得图像。使用离体OCT成像在相应的动物和人（尸体）收获组织节段中再现体内结果。对这些节段进行组织学检查以进行比较。以高分辨率（8 μm）显示猪颈总动脉OCT成像节段以及患者岩部和海绵窦颅内颈动脉的结构组成。体内图像与离体获得的图像相同，证明了血管内OCT器械的成像能力。OCT图像与组织切片后获得的图像具有良好的相关性，并在体内可视化了层状血管结构。神经血管内OCT成像在临床应用中是可行的，并且可以以高分辨率检测动脉节段的结构。了解非病变动脉中的OCT成像对于建立解释病理过程所需的基线结果非常重要。这允许在不需要切除和处理的情况下获得血管组织的神经血管内光学活检。
Intravascular optical coherence tomography (OCT) is a recently developed optical imaging technique that provides high-resolution cross-sectional in situ images from intact tissue based on tissue reflectance of near-infrared or infrared light. To report on the feasibility of neuroendovascular OCT imaging and compare the neuroendovascular OCT findings with histology in nondiseased vessels in an animal, cadaveric, and clinical study. Catheter-based in vivo endovascular OCT imaging was performed in the common carotid arteries of 2 pigs and in the intracranial carotid arteries of 3 patients. The endovascular OCT device was delivered to the desired location via groin access and using standard endovascular procedures. Images were obtained via rotational and translational scanning using external motors. In vivo findings were reproduced using ex vivo OCT imaging in corresponding animal and human (cadaveric) harvested tissue segments. These segments underwent histological examination for comparison. The structural compositions of the OCT-imaged segments of the common carotid arteries in pigs as well as the petrous and cavernous intracranial carotid arteries in patients were visualized at high resolution (8 μm). The in vivo images were identical to those obtained ex vivo, demonstrating the imaging capabilities of the endovascular OCT device. The OCT images correlated well with the images obtained after histological sectioning and visualized in vivo the laminar vascular structure. Neuroendovascular OCT imaging is feasible for clinical use and can detect with high resolution the structure of arterial segments. Understanding OCT imaging in nondiseased arteries is important in establishing baseline findings necessary for interpreting pathological processes. This allows neuroendovascular optical biopsies of vascular tissue to be obtained without the need for excision and processing.