Hind-limb regeneration in the Dwarf African Clawed Frog, Hymenochirus boettgeri (Anura: Pipidae)
Hind-limb regeneration in the Dwarf African Clawed Frog, Hymenochirus boettgeri (Anura: Pipidae)
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DOI：
10.2307/1565922
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发表时间：
2002-12-01
影响因子：
0.8
通讯作者：
Hall, BK
中科院分区：
文献类型：
--
作者：
Girvan, JE;Olson, WM;Hall, BK
两栖动物再生失去或截肢的肢体的能力差异很大。为了更好地了解影响再生能力的系统发育和个体发育因素，首次描述了膜手鲶蝌蚪的再生能力，然后与近亲非洲爪蟾的数据进行了比较。H. Boettgeri在52 - 59期在两个不同的水平沿着肢体的近端-远端轴（在膝关节和踝关节）截肢。让蝌蚪恢复15或20天，在此期间检查再生量。通过外部形态学和整体组织学染色进行观察。膜手龙，像X一样。laevis和实际上像大多数无尾类动物一样，随着其通过变态的进展，逐渐失去再生后肢结构的能力，并且这种损失发生在近端-远端方向;再生能力保留更长的时间，远端。膜手龙和爪蟾显示出相似的肢体和手指再生模式。在这两个pipid物种，再生能力的丧失似乎与后肢骨化的发生相关，尽管这一事件的时间在两个物种之间不同。
The ability to regenerate lost or amputated limbs varies greatly among amphibians. To gain a better understanding of phylogenetic and ontogenetic factors influencing regenerative ability regeneration in tadpoles of Hymenochirus boettgeri is described for the first time, then compared to data from closely related Xenopus laevis. Hind limbs of tadpoles of H. boettgeri were amputated at two different levels along the proximo-distal axis of the limb (at the knee and ankle joints), at stages ranging from 52 to 59. Tadpoles were allowed to recover for 15 or 20 days, at which times the amount of regeneration was examined. Observations were made through external morphology and whole-mount histological staining. Hymenochirus, like X. laevis and indeed like most anurans, gradually loses the ability to regenerate hind-limb structures as it progresses through metamorphosis, and this loss occurs in a proximo-distal direction; the ability to regenerate is retained longer, distally. Hymenochirus and Xenopus show similar patterns of limb and digit regeneration. In both pipid species, loss of regenerative ability appears to be correlated with the onset of ossification in the hind limb, although timing of this event differs between the two species.