Expression of matrix metalloproteinase and its tissue inhibitor in haemangioma
Expression of matrix metalloproteinase and its tissue inhibitor in haemangioma
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基质金属蛋白酶及其组织抑制剂在血管瘤中的表达
DOI：
10.1007/s11596-009-0516-3
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
S. Shan
中科院分区：
文献类型：
--
作者：
Shan Zhong;Guohua Yang;C. Xia;Zhang Duanlian;S. Shan
本研究采用免疫组织化学方法检测基质金属蛋白酶-2（MMP-2）和金属蛋白酶组织抑制剂-2（TIMP-2）在血管瘤组织中不同时期的表达，探讨MMP-2和TIMP-2在血管瘤发生、发展和退变中的作用机制。收集武汉大学人民医院病理科2000 - 2006年收治的皮肤毛细血管瘤石蜡包埋标本50例。常规HE染色，免疫组化S-P法检测增殖细胞核抗原（PCNA）。根据Mulliken分级标准和PCNA表达模式进行分级。应用免疫组化S-P法检测MMP-2、TIMP-2在皮肤毛细血管瘤增生期、退变期及正常皮肤组织中的表达。结合VIII因子相关抗原表达的检测，证实血管瘤组织中MMP-2和TIMP-2表达的细胞为血管内皮细胞。采用HPIAS-1000图像分析系统对MMP-2和TIMP-2的表达进行定量分析，用SPSS11.5软件对平均吸光度（A）和阳性颗粒面积率进行单因素方差分析（107）和SNK（q）检验。结果表明：（1）50例血管瘤标本中，26例为增生性血管瘤，24例为退行性血管瘤;（2）MMP-2在正常血管内皮细胞、结缔组织胞浆及血管周围细胞外基质中表达较弱。增生组MMP-2的表达明显高于退变组和对照组（正常皮肤）（P<0.05），后两组间差异无统计学意义;（3）TIMP-2在正常组织、退变血管内皮细胞、结缔组织胞浆及血管周围细胞外基质中高表达。TIMP-2在增殖期的表达水平明显低于退变期（P<0.05），增殖期TIMP-2的表达水平与退变期和正常组织相比差异有统计学意义（P<0.05）。结论：在血管瘤增生期，MMP-2可促进血管瘤内皮细胞过度增殖，而在血管瘤退变期，TIMP-2可抑制血管瘤内皮细胞增殖。这两种物质在血管瘤的发生、发展和变性中起重要作用。
SummaryThe action mechanism of matrix metalloproteinases-2 (MMP-2) and tissue inhibitor of metalloproteinases-2 (TIMP-2) in the genesis, development and degeneration of haemangioma was investigated by detecting their expression in the tissue of haemangioma in different phases by using the immunohistochemistry. Fifty paraffin-embedded specimens of skin capillary haemangioma were collected, which were documented in the Department of Pathology, Renmin Hospital of Wuhan University from 2000 to 2006. All samples were stained by regular HE method, and proliferative cell nuclear antigen (PCNA) was tested by immunohistochemical S-P method. The samples were classified according to the Mulliken criteria and the expression pattern of PCNA. Immunohistochemical S-P method was applied to detect the expression of MMP-2 and TIMP-2 in proliferative and degenerative phases of cutaneous capillary haemangioma, and in normal skin tissues. In combination with the detection of the expression of factor VIII-related antigen, it was verified that in haemangioma tissues, the cells expressing MMP-2 and TIMP-2 were vascular endothelial cells. The MMP-2 and TIMP-2 expression was quantitatively analyzed by image analysis system (HPIAS-1000), and one-way ANOVA(107) and SNK(q) test were done to analyze average absorbance (A) and positive area rate of immunohistochemically positive particles by using SPSS11.5. The results showed: (1) Among 50 samples of haemangioma, there were 26 proliferative haemangiomas, and 24 degenerative haemangiomas, respectively; (2) The expression of MMP-2 was weak in normal vascular endothelial cells, cytoplasm of connective tissues and extracellular matrix around blood vessels. The expression of MMP-2 in proliferative group was significantly higher than in degenerative group and control group (normal skin) (P<0.05), but there was no statistically significant difference between the latter two groups; (3) TIMP-2 was highly expressed in normal tissues, degenerative vascular endothelial cells, cytoplasm of connective tissues and extracellular matrix around blood vessels. The expression level of TIMP-2 in proliferative phase was significantly lower than in degenerative phase (P<0.05), and the expression of TIMP-2 in proliferative phase was significantly different from that in degenerative phase and normal tissues (P<0.05). It was concluded that in proliferative phase of haemangioma, MMP-2 may promote over-proliferation of endothelial cells of haemangioma, and in degenerative phase, TIMP-2 can inhibit the proliferation of endothelial cells of haemangioma. The two substances play important roles in the genesis, development and degeneration of haemangiomas.
影响因子：
4.7
作者：
R. Tunuguntla;D. Ripley;Q. Sang;N. Chegini
通讯作者：
R. Tunuguntla;D. Ripley;Q. Sang;N. Chegini