Intrapancreatic Parenchymal Injection of Cells as a Useful Tool for Allowing a Small Number of Proliferative Cells to Grow In Vivo.
Intrapancreatic Parenchymal Injection of Cells as a Useful Tool for Allowing a Small Number of Proliferative Cells to Grow In Vivo.
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DOI：
10.3390/ijms18081678
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发表时间：
2017-08-02
影响因子：
5.6
通讯作者：
Inada E
中科院分区：
文献类型：
--
作者：
Sato M;Saitoh I;Murakami T;Kubota N;Nakamura S;Watanabe S;Inada E
将细胞如肿瘤细胞和诱导多能干（iPS）/胚胎干（ES）细胞体内接种到免疫受损小鼠中已被认为是评价它们增殖或分化成源自三个生殖细胞层的各种细胞类型的潜力的有力技术。皮下移植和肾包膜下移植已被广泛用于此目的，但也有一些缺点，如需要大量的肿瘤细胞接种和成瘤失败的频繁。因此，已经探索了移植到其他部位，包括睾丸内或肌内移植以及移植到耳蜗、肝脏或唾液腺中。在这项研究中，我们发现胰腺实质内注射细胞有助于使少量细胞（约15 × 103个细胞或约30个细胞团μL−1·site−1）增殖，有时分化为各种类型的细胞。它只需要在背部皮肤上手术暴露胰腺，随后在基于解剖显微镜的观察下使用口件控制的玻璃微量移液器向胰腺实质注射细胞。我们现在将这项技术命名为“胰腺实质细胞移植（IPPCT）”，这将是有用的，特别是当只有少量细胞或集落可用时。
In vivo inoculation of cells such as tumor cells and induced pluripotent stem (iPS)/embryonic stem (ES) cells into immunocompromised mice has been considered as a powerful technique to evaluate their potential to proliferate or differentiate into various cell types originating from three germ cell layers. Subcutaneous grafting and grafting under the kidney capsule have been widely used for this purpose, but there are some demerits such as the requirement of a large number of tumor cells for inoculation and frequent failure of tumorigenesis. Therefore, grafting into other sites has been explored, including intratesticular or intramuscular grafting as well as grafting into the cochleae, liver, or salivary glands. In this study, we found that intrapancreatic parenchymal injection of cells is useful for allowing a small number of cells (~15 × 103 cells or ~30 cell clumps μL−1·site−1) to proliferate and sometimes differentiate into various types of cells. It requires only surgical exposure of the pancreas over the dorsal skin and subsequent injection of cells towards the pancreatic parenchyma under dissecting microscope-based observation using a mouthpiece-controlled glass micropipette. We now name this technology “intrapancreatic parenchymal cell transplantation (IPPCT)”, which will be useful, especially when only a small number of cells or colonies are available.
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影响因子：
3.7
作者：
Gropp M;Shilo V;Vainer G;Gov M;Gil Y;Khaner H;Matzrafi L;Idelson M;Kopolovic J;Zak NB;Reubinoff BE
通讯作者：
Reubinoff BE
影响因子：
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作者：
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通讯作者：
Ogura, Atsuo
影响因子：
5.2
作者：
Blum, Barak;Benvenisty, Nissim
通讯作者：
Benvenisty, Nissim
影响因子：
3.8
作者：
Choo, ABH;Padmanabhan, J;Oh, SKW
通讯作者：
Oh, SKW
影响因子：
--
作者：
Gertow, Karin;Przyborski, Stefan;Ahrlund-Richter, Lars
通讯作者：
Ahrlund-Richter, Lars