Role of monochromatic light on daily variation of clock gene expression in the pineal gland of chick
Role of monochromatic light on daily variation of clock gene expression in the pineal gland of chick
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单色光对雏鸡松果体时钟基因表达日变化的作用
DOI：
10.1016/j.jphotobiol.2016.09.020
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发表时间：
2016-11-01
影响因子：
5.4
通讯作者：
Chen, Yaoxing
中科院分区：
文献类型：
--
作者：
Jiang, Nan;Wang, Zixu;Chen, Yaoxing
鸟类的松果体是一个主时钟，它可以接收外部的光信号，并将其转化为输出节奏。为了阐明光波长的变化是否会影响鸡松果体的昼夜节律表达，总共240只Arbor Acre雄性肉仔鸡暴露于白色光（WL）、红光（RL）、绿色光（GL）和蓝光（BL）。光照2周后，检测松果体7个核心时钟基因的昼夜表达和血浆褪黑素水平。结果显示，在单色光照射后，除RL有破坏cCry 2节律的趋势外，所有时钟基因mRNA的24 h谱均保持昼夜节律振荡。与WL相比，BL使cCry 1、cCry 2、cPer 2和cPer 3的峰期提前0.1- 1.5h，使cClock、cBmal 1和cBmal 2的峰期延迟0.2 ~ 0.8h。RL使除cClock和cPer 2外的所有时钟基因提前0.3-2.1 h，而GL使除cBmal 2外的所有时钟基因延迟0.5-1.5 h。同时，GL增加了负时钟基因的振幅和信号，降低了负时钟基因的振幅和信号，而RL则相反。尽管GL和RL均使血浆褪黑素峰值相提前，但GL组褪黑素水平显著升高，RL组褪黑素水平显著降低，这一趋势与单色光下正时钟基因mRNA水平的变化一致，而与负时钟基因的变化相反。因此，我们推测GL可能会增强正时钟基因的表达，从而导致褪黑素的合成，而RL可能会增强负基因的表达，抑制褪黑素的合成。(C)© 2016 Elsevier B. V.版权所有。
The avian pineal gland is a master clock that can receive external photic cues and translate them into output rhythms. To clarify whether a shift in light wavelength can influence the circadian expression in chick pineal gland, a total of 240 Arbor Acre male broilers were exposed to white light (WL), red light (RL), green light (GL) or blue light (BL). After 2 weeks light illumination, circadian expressions of seven core clock genes in pineal gland and the level of melatonin in plasma were examined. The results showed after illumination with monochromatic light, 24 h profiles of all clock gene mRNAs retained circadian oscillation, except that RL tended to disrupt the rhythm of cCry2. Compared to WL, BL advanced the acrophases of the negative elements (cCry1, cCry2, cPer2 and cPer3) by 0.1-1.5 h and delayed those of positive elements (cClock, cBmal1 and cBmal2) by 02-0.8 h. And, RL advanced all clock genes except cClock and cPer2 by 0.3-2.1 h, while GL delayed all clock genes by 0.5-1.5 h except cBmal2. Meanwhile, GL increased the amplitude and mesor of positive and reduced both parameters of negative clock genes, but RL showed the opposite pattern. Although the acrophase of plasma melatonin was advanced by both GL and RL, the melatonin level was significantly increased in GL and decreased in RL This tendency was consistent with the variations in the positive clock gene mRNA levels under monochromatic light and contrasted with those of negative clock genes. Therefore, we speculate that GL may enhance positive clock genes expression, leading to melatonin synthesis, whereas RL may enhance negative genes expression, suppressing melatonin synthesis. (C) 2016 Elsevier B.V. All rights reserved.