The H+/K+-ATPase (proton) pump is expressed in human laryngeal submucosal glands
The H+/K+-ATPase (proton) pump is expressed in human laryngeal submucosal glands
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DOI：
10.1097/00005537-200311000-00013
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发表时间：
2003-11-01
期刊：
影响因子：
2.6
通讯作者：
Radosevich, JA
中科院分区：
文献类型：
--
作者：
Altman, KW;Haines, GK;Radosevich, JA
目的/假设：近年来，胃食管和咽喉反流病的诊断和治疗显著增加。喉对LPRD的作用高度敏感，对质子泵抑制剂药物治疗也有类似的反应。该研究的假设是质子泵活性存在于人喉中，并在正常和/或病理喉组织中发挥功能作用。研究设计：病理学研究。研究方法：作为人类受试者委员会豁免方案的一部分，获得两个新鲜人尸体喉（一个男性和一个女性喉），并进行福尔马林固定和石蜡包埋。还获得了库存人胃组织，用作比较阳性和阴性对照标本。用与H+/K+-ATP酶（质子）泵的α和β亚基反应的单克隆抗体对切片进行免疫染色。审查标本的染色模式和强度。结果如下：胃壁细胞（已知产生胃酸）对质子泵的α和β亚基都表现出强阳性染色。与壁细胞形态不一致的胃细胞中没有染色。在人喉中，质子泵的α和β单克隆抗体在浆液细胞和小浆液粘液腺导管中具有强局灶性和相同的染色模式。喉上皮中存在可变染色，认为与组织处理导致的伪影染色一致。结论：人喉粘膜下腺体的浆液细胞和导管中存在H+/K+-ATP酶（质子）泵。质子泵抑制剂药物治疗可能在人喉的浆液粘液腺中具有作用部位，可能与治疗慢性喉炎伴或不伴咽喉反流病的患者相关。
Objectives/Hypothesis: Diagnosis and treatment of gastroesophageal and laryngopharyngeal reflux disease has significantly increased over recent years. The larynx is highly sensitive to the effects of LPRD and is similarly responsive to proton pump inhibitor pharmacotherapy. The hypothesis of the study was that proton pump activity exists in the human larynx and plays a functional role in normal and/or pathological laryngeal tissue. Study Design: Pathological investigation. Methods: Two fresh human cadaveric larynges (one male and one female larynx) were obtained as part of an exempt protocol from the Human Subjects Committee and were formalin fixed and paraffin embedded. Banked human stomach tissue was also obtained for use as comparative positive and negative control specimens. Sections were immunostained with monoclonal antibodies reactive with both alpha and beta subunits of the H+/K+-ATPase (proton) pump. Specimens were reviewed for staining pattern and intensity. Results: Stomach parietal cells (known to produce gastric acid) exhibited strongly positive staining for both the alpha and beta subunits of the proton pump. There was no staining in stomach cells that were not morphologically consistent with the parietal cell. In the human larynx there were strong focal and identical staining patterns in the serous cells and ducts of the minor seromucinous glands by both alpha and beta monoclonals to the proton pump. There was variable staining in the laryngeal epithelium that was thought to be consistent with artifact staining resulting from tissue processing. Conclusion: The H+/K+-ATPase (proton) pump is present in serous cells and ducts of submucosal glands in the human larynx. Proton pump inhibitor pharmacotherapy may have a site of action in seromucinous glands of the human larynx, with possible relevance for patients treated for chronic laryngitis with or without laryngopharyngeal reflux disease.