EARLY gestational exposure to isoflurane causes persistent cell loss in the dentate gyrus of adult male rats.
EARLY gestational exposure to isoflurane causes persistent cell loss in the dentate gyrus of adult male rats.
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DOI：
10.1186/s12993-017-0132-5
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发表时间：
2017-12-26
期刊：
影响因子：
--
通讯作者：
Culley DJ
中科院分区：
文献类型：
--
作者：
Palanisamy A;Crosby G;Culley DJ
我们以前的研究表明，4小时的母体麻醉与异氟醚在妊娠早期大鼠导致空间记忆的成年雄性后代的缺陷。由于空间记忆主要是一种脑内介导的任务，我们提出的问题是，妊娠早期暴露于异氟烷是否会影响后代海马体的发育。在妊娠中期暴露于异氟烷的先前行为表征的成年雄性大鼠在4月龄时处死（N = 10和13，分别为对照组和异氟烷组），用于海马亚区的定量组织学。切片用甲酚紫染色，并由盲法观察者使用无偏体视学原理和光学分馏仪方法确定齿状回颗粒层和CA 1区锥体细胞层中的细胞总数。采用Student t检验分析数据; P < 0.05具有统计学意义。体视学检查显示，与对照组相比，异氟烷暴露的成年大鼠齿状回颗粒层中的细胞减少9%（分别为1，002，122 ± 84，870 vs. 1，091，829 ± 65，791;平均值±标准差，*P = 0.01）。相反，CA 1区的细胞数没有变化，两个区域的体积也没有变化。我们的研究结果表明，母亲异氟烷麻醉啮齿动物导致特定区域的细胞损失在成年雄性后代的海马。这些变化可能部分解释了在子宫内暴露于异氟烷的成年大鼠中报告的行为缺陷。
Our previous research showed that 4 h of maternal anesthesia with isoflurane during early gestation in pregnant rats leads to a deficit in spatial memory of adult male offspring. Because spatial memory is predominantly a hippocampally-mediated task, we asked the question if early gestational exposure to isoflurane affects development of the hippocampus in the offspring. Previously behaviorally characterized adult male rats that were exposed to isoflurane during second trimester were sacrificed at 4 months of age (N = 10 and 13, control and isoflurane groups, respectively) for quantitative histology of hippocampal subregions. Sections were stained with cresyl violet and the total number of cells in the granular layer of the dentate gyrus and the pyramidal cell layer in the CA1 region were determined by a blinded observer using unbiased stereological principles and the optical fractionator method. Data were analyzed using Student’s t test; P < 0.05 was accorded statistical significance. Stereological examination revealed 9% fewer cells in the granular layer of the dentate gyrus of isoflurane-exposed adult rats compared to controls (1,002,122 ± 84,870 vs. 1,091,829 ± 65,791, respectively; Mean ± S.D, *P = 0.01). In contrast, there were no changes in the cell number in the CA1 region, nor were there changes in the volumes of both regions. Our results show that maternal isoflurane anesthesia in rodents causes region-specific cell loss in the hippocampus of adult male offspring. These changes may, in part, account for the behavioral deficits reported in adult rats exposed to isoflurane in utero.
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影响因子：
6.4
作者：
Rizzi, Sabina;Carter, Lisa B.;Jevtovic-Todorovic, Vesna
通讯作者：
Jevtovic-Todorovic, Vesna
影响因子：
3
作者：
Clancy, Barbara;Kersh, Brandon;Finlay, Barbara L.
通讯作者：
Finlay, Barbara L.
影响因子：
8.8
作者：
Palanisamy A;Baxter MG;Keel PK;Xie Z;Crosby G;Culley DJ
通讯作者：
Culley DJ
影响因子：
8.8
作者：
Stratmann, Greg;Sall, Jeffrey W.;Dai, Ran
通讯作者：
Dai, Ran
影响因子：
5.3
作者：
Jevtovic-Todorovic, V;Hartman, RE;Wozniak, DF
通讯作者：
Wozniak, DF