Correlation between Cells-on-Chips materials and cell adhesion/proliferation focused on material’s surface free energy
Correlation between Cells-on-Chips materials and cell adhesion/proliferation focused on material’s surface free energy
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DOI：
10.1016/j.colsurfa.2018.12.059
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发表时间：
2019-03
期刊：
影响因子：
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通讯作者：
Hidetaka Ueno;Masatoshi Inoue;A. Okonogi;H. Kotera;Takaaki Suzuki
中科院分区：
文献类型：
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作者：
Hidetaka Ueno;Masatoshi Inoue;A. Okonogi;H. Kotera;Takaaki Suzuki
细胞表面反应，如材料表面特性和细胞增殖/粘附率是相关的，这种相关性可以用作缩短开发周期和降低选择用于生物实验的材料和生物实验仪器的成本的早期确定标准。在利用MEMS技术制备微流控器件Cells-on-Chips时，需要考虑支架材料的影响。由于片上细胞的构成材料种类繁多，因此需要针对特定环境和各种材料的高度通用性的早期判定标准。在本研究中，我们提出了利用表面自由能来确定构成片上细胞的脚手架材料的早期判定标准。表面自由能是由接触角计算得到的，测量方便且成本低廉。选择了6种材料，不考虑无机/有机和光敏/非光敏性质。当在每种材料上培养HeLa细胞时，观察到表面自由能与细胞增殖/粘附率之间的显著相关性。因此，材料的表面自由能适合作为选择片上电池组成材料的早期判定标准。与传统的评价标准相比，所提出的评价标准具有较高的通用性，可用于各种材料的评价。
Cell surface reactions such as material surface characteristics and cell proliferation/adhesion rates are correlated and this correlation can be used as early determination criteria for shortening the development period and reducing costs of selecting materials used for bio-experiments, and of bio-experimental instruments. When fabricating Cells-on-Chips, a microfluidic device fabricated using MEMS technology, the influence of the scaffold material should be considered. Since a wide variety of materials are used as constituent materials of Cells-on-Chips, highly versatile early determination criteria corresponding to a specific environment and various materials are needed. In this research, we propose the early determination criteria of scaffolding material composing Cells-on-Chips by using surface free energy, which is calculated from contact angle that can be measured easily and inexpensively. Six materials were selected, regardless of inorganic/organic and photosensitive/non-photosensitive properties. When HeLa cells were cultured on each material, a significant correlation was observed between surface free energy and cell proliferation/adhesion rates. Therefore, the surface free energy of the material is appropriate as the early determination criteria for selecting the constituent material of Cells-on-Chips. Compared with the conventional criteria, the proposed criteria have high versatility regardless of the kind of material and can be used for evaluation of various materials.