Current source-density analysis in the mushroom bodies of the honeybee (Apis mellifera carnica)
Current source-density analysis in the mushroom bodies of the honeybee (Apis mellifera carnica)
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蜜蜂蘑菇体中的电流源密度分析（Apis mellifera carnica）
DOI：
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发表时间：
1984
期刊：
影响因子：
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通讯作者：
P. Mobbs
中科院分区：
文献类型：
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作者：
P. Kaulen;J. Erber;P. Mobbs
摘要利用细胞外电生理技术分析了无脊椎动物中枢神经系统重要组成部分蘑菇体的信息处理过程。蘑菇体由多层平行的内在神经元组成，它们与外在的输入和输出神经元进行突触接触。固有神经元（约17万个/蘑菇体）的轴突直径非常小（0.1-1 μm），难以在细胞内记录其活动。为了分析其功能特性，我们在细胞外测量了其场电位。在连续深度间隔的电极轨迹上记录了蜜蜂蘑菇体不同部位的一系列平均诱发电位（AEPs）。这些电位由嗅觉、机械和视觉刺激引起。为了定位产生这些电位的突触区域，使用连续测量的诱发电位计算电流源密度（CSD）。沿电极轨迹的胞外空间的电导率在蘑菇体的花梗和花萼以及部分α叶中被发现是恒定的。CSD分析揭示了菌体源库分布的复杂模式。CSD分析检测到的电流汇和电流源与神经元的形态分布有高度的相关性。CSD分析表明，蘑菇体的输入和输出涉及多模态突触相互作用，而内部肯恩细胞的信息处理仅限于来自天线的感觉输入。电生理学结果与组织学结果的比较表明，蘑菇体的内在细胞在生理上不是一个均匀的群体。在固有神经元中，电流源和电流汇的明确区域可以被识别并归因于不同层的肯尼恩细胞。
SummaryInformation processing in the mushroom bodies which are an important part of most invertebrate central nervous systems was analysed by extracellular electrophysiological techniques. The mushroom bodies consist of layers of parallel intrinsic neurons which make synaptic contact with extrinsic input and output neurons. The intrinsic neurons (approximately 170,000/mushroom body) have very small axon diameters (0.1–1 μm) which makes it difficult to record their activity intracellularly. In order to analyse the functional properties of this neuropil field potentials were measured extracellularly.Series of averaged evoked potentials (AEPs) were recorded along electrode tracks at consecutive depth intervals in different parts of the mushroom bodies of the bee. These potentials were elicited by olfactory, mechanical and visual stimuli.In order to locate the synaptic areas generating these potentials, current source-densities (CSD) were calculated using the consecutively measured evoked potentials. The conductivities of the extracellular space along the electrode tracks in the pedunculus and calyx and in part of the alpha-lobe of the mushroom bodies were found to be constant.The CSD analysis reveals a complex pattern of source-sink distributions in the mushroom bodies. There is a high degree of correlation between current sinks and sources detected by CSD analysis and the morphological distribution of neurons.The CSD analysis shows that the inputs and outputs of the mushroom bodies involve multimodal synaptic interactions, whereas information processing in the intrinsic Kenyon-cells is limited to sensory inputs from the antenna.Comparison of the electrophysiological with the histological results shows that the intrinsic cells of the mushroom bodies are physiologically not a homogeneous group as is often proposed. Among the intrinsic neurons clearly defined areas of current sources and sinks can be identified and attributed to Kenyon-cells in different layers.