Suppression of red color development associated with anthocyanin accumulation in the epicarp of grape (Vitis labrusca x vinifera cv. Ruby Roman) berries caused by air temperature in daylight periods higher than 33 degrees C during maturation
Suppression of red color development associated with anthocyanin accumulation in the epicarp of grape (Vitis labrusca x vinifera cv. Ruby Roman) berries caused by air temperature in daylight periods higher than 33 degrees C during maturation
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葡萄（Vitis labrusca x vinifera cv. Ruby Roman）浆果成熟过程中白天气温高于 33°C 时，会抑制与花青素积累相关的红色发展
DOI：
10.1016/j.scienta.2021.110381
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发表时间：
2021
影响因子：
4.3
通讯作者：
Takashi Suzuki
中科院分区：
文献类型：
--
作者：
Kenichi Matsuda;Mei Gao-Takai;Ayaka Date;Takashi Suzuki
研究了高温对红宝石罗马葡萄(Vitis Labruscana×Vinifera)果实果皮花青素积累的影响。用高效液相色谱法从成熟浆果的外果皮组织中检测到17种花色苷。由于颜色表色密度与花色苷总含量之间存在很强的正相关关系(P<；0.005，R2=0.0.937)，因此，在不同温度下的果实成熟过程中，使用颜色表来追踪果实中花色苷的积累似乎是可能的。采用点式降温系统对果串进行降温处理，可在盛花后连续降温(0：00~24：00)、白天(6：00~18：00)降温、夜间(18：00~6：00)降温和不降温(对照)。连续降温后(降温后0~15天)，连续降温处理组的颜色密度值显著高于对照组(P<；0.05)。为了阐明有或无日光温度区对外果皮花青素积累的影响，分别计算了日光和暗期在<；21、21-24、24-27、27-30、30-33、33-36和36°C≤的每个温区的暴露时间百分比。∆时间百分比(与对照的曝光时间百分比的差值)与∆色度(与对照的色度差值)在<；30°C的温度范围内呈正相关，而在30°C的≤温度范围内转为负相关。在白天27-30℃(正)和33-36℃(负)的温度范围内，花色苷积累开始受到抑制的阈值存在于30-33℃之间，相关系数达到显著水平(P<0.05)。
The effect of high air temperature on the anthocyanin accumulation in the epicarp of 'Ruby Roman' grape (Vitis labruscana×vinifera)berries was examined. Seventeen types of anthocyanin were detected from the epicarp tissue of the matured berries using HPLC. Since a strong positive correlation (P< 0.005,R2= 0.937) was confirmed between the degree of color density evaluated using a color chart and the total content of anthocyanins quantified separately, it seems possible to trace anthocyanin accumulation using the color chart on each berry during maturation at different air temperatures. Four conditions were designed for cooling the fruit clusters with a spot-cooling system, which can keep air temperature 4–5°C lower for 60–70 days after the full–bloom, i.e. consecutive (0:00–24:00) cooling, daytime (6:00–18:00) cooling, nighttime (18:00–6:00) cooling and no–cooling (control). The degree of color density showed statistically higher (P< 0.05) values in the consecutive–cooling treatment than that of the control through the post–cooling duration (0–15 days after cooling). To clarify the effect of temperature zones with or without daylight on the anthocyanin accumulation in the epicarp, percentage of exposure time to each temperature zone of < 21, 21–24, 24–27, 27–30, 30–33, 33–36 and 36°C ≤ were calculated for both daylight and dark periods, separately. Correlations of the ∆ time% (the amount of difference in percentage of exposure time from the control) with the ∆ color degree (the amount of difference in degree of color density from the control) in the daylight period were positive at temperature zones of < 30°C, but turned to negative at those of 30°C ≤. The fact that correlation coefficient was statistically significant (P< 0.05) at the temperature zones of 27–30°C (positive) and 33–36°C (negative) in the daylight period shows that the threshold where the anthocyanin accumulation starts to be suppressed exists within 30–33°C.