Circadian rhythms in behavior and clock gene expressions in the brain of mice lacking histidine decarboxylase
Circadian rhythms in behavior and clock gene expressions in the brain of mice lacking histidine decarboxylase
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DOI：
10.1016/j.molbrainres.2004.02.015
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发表时间：
2004-05-19
期刊：
影响因子：
--
通讯作者：
Honma, K
中科院分区：
文献类型：
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作者：
Abe, H;Honma, S;Honma, K
为了阐明组胺在生物钟系统中的功能作用，对缺乏组氨酸脱羧酶的小鼠(HDC-/-小鼠)的行为和脑内时钟基因表达的昼夜节律进行了研究。分别在明暗循环(LD)和恒定黑暗(DD)条件下记录大鼠跑轮和自发活动。用原位杂交法检测LD和DID刺激下大鼠视交叉上核(SCN)、大脑皮层和纹状体mPer1、mpET-2和mBMALI基因表达节律。HDC-/-小鼠在LD和DD下的活动水平均低于野生型，与活动类型(车轮跑和自发运动)无关。在DD下，HDC-/-小鼠的自由奔跑期明显长于野生型小鼠。SCN中mPer1、mPer2和mBMAL1基因表达的24小时谱无明显差异。相比之下，HDC-/-小鼠大脑其他区域，如皮质和纹状体的mPer1和mPer2的mPer1和mPer2基因节律显著中断。这些结果表明，组胺参与了昼夜节律系统，特别是在SCN从行为到起搏器的输出通路或反馈通路中，并且SCN外脑区的mper基因在行为节律的表达中起着重要作用。(C)2004爱思唯尔B.V.保留所有权利。
To clarify functional roles of histamine in the circadian clock system, circadian rhythms of behavior and clock gene expression in the brain were examined in the mouse lacking histidine decarboxylase (HDC - / - mouse). Wheel-running and spontaneous locomotion were recorded under light-dark cycle (LD) and constant darkness (DD). mPer1, mPet-2 and mBMALI mRNA expression rhythms under LD and DID were measured in the suprachiasmatic nucleus (SCN), cerebral cortex and striatum by in situ hybridization. The activity levels under LD and DD in the HDC - / - mice were lower than that in the wild type regardless of activity types (wheel-running and spontaneous locomotion). The free-running period under DD was significantly longer in the HDC - / - mice than in the wild type. The 24-h profiles of mPer1, mPer2 and mBMALl mRNA expressions in the SCN were not different between the two genotypes. By contrast, the mPer1 and mPer2 mRNA rhythms in the other brain areas such as the cortex and striatum were significantly disrupted in the HDC - / - mice. These results suggest that histamine is involved in the circadian system especially in the output pathway or feedback route from behavior to the pacemaker in the SCN, and that mPer genes in the brain areas outside the SCN play an important role in the expression of behavioral rhythm. (C) 2004 Elsevier B.V. All rights reserved.