NaF potentiates a K+-selective ion channel in G292 osteoblastic cells
NaF potentiates a K+-selective ion channel in G292 osteoblastic cells
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DOI：
10.1007/s002329900021
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发表时间：
1996-02-01
影响因子：
2.4
通讯作者：
Davidson, RM
中科院分区：
文献类型：
--
作者：
Gofa, A;Davidson, RM
临床研究已经确定，氟化钠增加骨中的矿物质含量，尽管其骨诱导作用的细胞机制尚不清楚。由于氟的代谢效应与离子通量和膜电位的改变有关，我们使用膜片钳记录技术来研究成骨细胞对氟化钠的电生理反应。在这些实验中，我们表明，氟化钠增加了73 pS钾离子选择性通道的振幅和P-开放。NaF的作用依赖于细胞外Ca 2+，并可被钙通道阻滞剂的组合所阻断，表明通道活性的增强依赖于外部钙。因为所有的补丁都在细胞附着配置，氟化钠的影响可能是间接的。虽然潜在的细胞机制仍不清楚，我们的研究结果表明，通过第二信使级联的钙和/或钾选择性通道的活动可能介导的骨细胞对无机氟的反应中涉及的许多早期事件。
Clinical studies have established that NaF increases mineral content in bone, although the cellular mechanisms underlying its osteoinductive effects remain unclear. Because metabolic effects of fluoride have been linked to ion flux and alterations in membrane potential, we used patch-clamp recording techniques to examine the electrophysiological response of osteoblastic cells to NaF. In these experiments, we show that NaF increased the amplitude and P-open of a 73 pS potassium-selective ion channel. The effect of NaF depended on extracellular Ca2+ and could be blocked by a combination of calcium-channel blocking agents, suggesting that potentiation of channel activity was dependent on external calcium. Because all patches were in the cell-attached configuration, the effect of NaF was presumably indirect. Although the underlying cellular mechanisms remain unclear, our findings suggest that activity of calcium and/or potassium-selective channels via second messenger cascades may mediate many of the early events involved in the response of bone cells to inorganic fluoride.