Localization of adenovirus-encoded DNA replication proteins in the nucleus by immunogold electron microscopy.
Localization of adenovirus-encoded DNA replication proteins in the nucleus by immunogold electron microscopy.
复制标题
通过免疫金电子显微镜定位腺病毒编码的 DNA 复制蛋白在细胞核中的位置。
DOI：
10.1099/0022-1317-71-12-2847
复制
发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Kitchingman,GR
中科院分区：
文献类型：
--
作者：
Murti,KG;Davis,DS;Kitchingman,GR
通过免疫金电子显微镜研究了三种腺病毒编码的DNA复制蛋白在人293细胞核中的分布。受感染的细胞核含有四种形态不同的内含物。它们是高电子密度颗粒（I型）、中等电子密度的致密纤维颗粒团块（II型）、低电子密度的丝状团块（III型）和大的多边形晶体（IV型）。在免疫金标记研究中，腺病毒单链 DNA 结合蛋白 (DBP) 的抗体和单链 DNA 的抗体显示出与 III 型包涵体的广泛结合。腺病毒 DNA 聚合酶 (AdPol) 和末端蛋白 (TP) 的抗体主要标记 II 型包含物。双免疫金标记研究检测到 III 型内含物中的 AdPol 和 TP 含量较低，而 II 型内含物中的 DBP 含量较低。 DNA复制蛋白的选择性分布表明II型和III型夹杂物代表两种功能不同的实体，可能涉及腺病毒DNA复制的两个不同方面，即链起始和延伸。
The distribution of three adenovirus-encoded DNA replication proteins in the nucleus of human 293 cells was studied by immunogold electron microscopy. The infected nuclei contained four morphologically distinct inclusions. They were highly electron-dense granules (type I), compact fibrogranular masses of medium electron density (type II), filamentous masses of low electron density (type III) and large polygonal crystals (type IV). In immunogold labelling studies, antibodies to the adenovirus single-stranded DNA-binding protein (DBP) and antibodies to single-stranded DNA showed extensive binding to the type III inclusions. The antibodies to the adenovirus DNA polymerase (AdPol) and terminal protein (TP) predominantly labelled type II inclusions. Double immunogold labelling studies detected low levels of AdPol and TP in type III inclusions and DBP in type II inclusions. The selective distribution of DNA replication proteins suggests that the type II and III inclusions represent two functionally different entities that may be involved in two different aspects of adenovirus DNA replication, i.e. chain initiation and elongation.