In Vivo Antibacterial Efficacy of Nitric Oxide-Releasing Hyperbranched Polymers against Porphyromonas gingivalis.
In Vivo Antibacterial Efficacy of Nitric Oxide-Releasing Hyperbranched Polymers against Porphyromonas gingivalis.
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DOI：
10.1021/acs.molpharmaceut.9b00671
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发表时间：
2019-06
影响因子：
4.9
通讯作者：
Lei Yang;L. Jing;Y. Jiao;Lufei Wang;J. Marchesan;S. Offenbacher;M. Schoenfisch
中科院分区：
文献类型：
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作者：
Lei Yang;L. Jing;Y. Jiao;Lufei Wang;J. Marchesan;S. Offenbacher;M. Schoenfisch
NO释放超支化聚合物的体内抗菌活性进行了评价对牙龈卟啉单胞菌，一个关键的口腔病原体与牙周炎，使用小鼠皮下腔室模型。将递增剂量的NO释放聚合物（1.5、7.5和37.5mg/kg）施用到牙龈卟啉单胞菌感染的室中，每天一次，持续3天。在第4天收集腔室液体，微生物学评价表明具有剂量依赖性杀菌作用。特别地，37.5mg/kg（1170 μg NO/kg）的NO释放聚合物实现了完全的细菌根除（细菌活力降低>6-log），证明了比阿莫西林（细菌活力降低104-log）（一种常用的抗生素）更大的功效。时间杀灭测定进一步揭示，最大剂量（37.5 mg/kg; 1170 μg NO/kg）仅在单次剂量后导致牙龈卟啉单胞菌的103-log杀灭。基于这些结果，释放NO的超支化聚合物的潜在临床应用似乎是有希望的，特别是对于口腔健康应用。
The in vivo antibacterial activity of NO-releasing hyperbranched polymers was evaluated against Porphyromonas gingivalis, a key oral pathogen associated with periodontitis, using a murine subcutaneous chamber model. Escalating doses of NO-releasing polymers (1.5, 7.5, and 37.5 mg/kg) were administered into a P. gingivalis-infected chamber once a day for 3 days. Chamber fluids were collected on day 4, with microbiological evaluation indicating a dose-dependent bactericidal action. In particular, NO-releasing polymers at 37.5 mg/kg (1170 μg of NO/kg) achieved complete bacterial eradication (>6-log reduction in bacterial viability), demonstrating greater efficacy than amoxicillin (∼4-log reduction in bacterial viability), a commonly used antibiotic. Time-kill assays further revealed that largest dose (37.5 mg/kg; 1170 μg of NO/kg) resulted in ∼3-log killing of P. gingivalis after only a single dose. Based on these results, the potential clinical utility of NO-releasing hyperbranched polymers appears promising, particularly for oral health applications.