Detection and characterization of quantitative trait loci for coleoptile elongation under anaerobic conditions in rice
Detection and characterization of quantitative trait loci for coleoptile elongation under anaerobic conditions in rice
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DOI：
10.1080/1343943x.2020.1740600
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发表时间：
2020-03-19
影响因子：
2.5
通讯作者：
Kato, Yoichiro
中科院分区：
文献类型：
--
作者：
Nishimura, Tomio;Sasaki, Kazuhiro;Kato, Yoichiro
淹水稻田氧气浓度低导致湿直播水稻出苗率低。我们的目标是寻找与厌氧条件下胚芽鞘伸长相关的新的基因组区域，以提高幼苗的成苗率。本文对以稻作类型的Koshihikari代换而成的染色体片段代换系(CSSL)进行了评价。在30℃下培养6天后，测量芽鞘长度。在CSSL中，只有SL2111的芽鞘长度(23.9 mm)显著长于IR的(14.3 mm)。从基因型和表型分析中，在3号染色体上检测到一个新的控制无氧条件下芽鞘伸长的QTL，命名为qACE3.1。为了探讨qACE3.1的分子机制，我们对参与淀粉降解和发酵的编码酶基因的表达水平进行了分析。在SL2111胚芽鞘中，丙酮酸脱羧酶的表达水平显著高于IR胚芽鞘，而乙醇脱氢酶的表达水平则显著低于IR。相反，与淀粉降解相关的基因表达水平没有发现差异。这些结果表明，qACE3.1可能特异性地影响发酵代谢。此外，我们还评价了qACE3.1在水田淹水条件下对育苗的影响。
Low oxygen concentrations in flooded paddy fields cause poor seedling establishment in wet direct seeded rice. We aimed to identify novel genomic regions associated with coleoptile elongation under anaerobic conditions in order to improve seedling establishment. Chromosome segment substitution lines (CSSLs), substituted with Koshihikari (Japonica-type) in the IR 64 genetic background (Indica-type) were evaluated. These lines were imbibed with a hydroponic solution containing Oxyrase to create a stable anaerobic condition and coleoptile lengths were measured six days after incubation at 30 degrees C. Among the CSSLs, only SL2111 had a significantly longer coleoptile (23.9 mm) than that of IR 64 (14.3 mm). From genotype and phenotype analyses, a novel QTL, referred to as qACE3.1, for coleoptile elongation under anaerobic conditions was detected on chromosome 3. To explore the molecular mechanism of qACE3.1, the expression levels of genes encoding enzymes involved in starch degradation and fermentation were assessed. In SL2111 coleoptiles, the expression levels of pyruvate decarboxylase were significantly higher than in IR 64 coleoptiles whereas those of alcohol dehydrogenase were lower. In contrast, no differences were observed in the expression levels of genes associated with starch degradation. These results imply that qACE3.1 may specifically affect fermentative metabolism. In addition, we evaluated the impact of qACE3.1 on seedling establishment under flooded conditions in a paddy field.