Novel porcine rotavirus of genotype P[27] shares new phylogenetic lineage with G2 porcine rotavirus strain
Novel porcine rotavirus of genotype P[27] shares new phylogenetic lineage with G2 porcine rotavirus strain
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DOI：
10.1016/j.virol.2006.12.004
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发表时间：
2007-05-10
期刊：
影响因子：
3.7
通讯作者：
Ushijima, Hiroshi
中科院分区：
文献类型：
--
作者：
Khamrin, Pattara;Maneekarn, Niwat;Ushijima, Hiroshi
2000年6月至2001年7月在泰国清迈省进行的猪轮状病毒感染流行病学调查中，从一头7周龄仔猪中分离到一株新的猪轮状病毒（PoRV）CMP 034株。序列分析表明，VP 4基因的氨基酸序列同源性较低，为56.7%~ 76.6%，而VP 8 ~* 部分的同源性为41.8%~ 69.9%，与目前已知的26种P基因型一致。系统发育分析表明，CMP 034与其他26种P基因型的亲缘关系较远，属于独立的分支。VP 7基因与PoRV G2型参考株34461-4的氨基酸同源性最高（94.7%），与人G2型轮状病毒的同源性较低（87.7%~ 88.0%）。VP 7基因的系统发育分析揭示了G2轮状病毒株基于宿主来源的两个主要谱系。PoRV毒株CMP 034与G2样PoRV毒株34461-4在一个新的谱系中分离地聚簇，该谱系不同于主要的G2人类谱系。此外，菌株CMP 034显示猪样VP 6和具有亚群I特异性的NSP 5/6，同时携带具有某些遗传群B人样特征的NSP 4。这些发现提供了证据，表明CMP 034应被视为新的VP 4基因型P[27]。(c)2006年爱思唯尔公司All rights reserved.
A novel and unusual strain of porcine rotavirus (PoRV) CMP034 was isolated from a 7-week-old piglet during the epidemiological survey of porcine rotavirus infection in Chiang Mai province, Thailand from June 2000 to July 2001. Molecular characterization of gene VP4 by sequence analysis showed a low level of amino acid sequence identity, ranging from 56.7% to 76.6%, while comparison of VP8* portion showed 41.8% to 69.9% identity, with :he 26 P genotypes recognized to date. Phylogenetic analysis of the VP4 sequence revealed that CMP034 was only distantly related to the other 26 P genotypes and was located in a separate branch. Sequence analysis of gene VP7 showed the highest level of amino acid identity (94.7%) with the PoRV G2-like reference strain 34461-4 but a lower level of identity with those of human G2 rotaviruses, ranging from 87.7% to 88.0%. Phylogenetic analysis of gene VP7 revealed two major lineages among G2 rotavirus strains based on the host origin. PoRV strain CMP034 clustered e elusively with G2-like PoRV strain 34461-4 in a novel lineage that is distinct from the major G2 human lineage. Moreover, strain CMP034 displayed a porcine-like VP6 and NSP5/6 with subgroup I specificity, while bearing an NSP4 with some genetic group B human-like characteristics. These findings provide evidence that CMP034 should be considered as a novel VP4 genotype P[27]. (c) 2006 Elsevier Inc. All rights reserved.