Chromosomal amplifications, 3q gain and deletions of 2q33-q37 are the frequent genetic changes in cervical carcinoma.
Chromosomal amplifications, 3q gain and deletions of 2q33-q37 are the frequent genetic changes in cervical carcinoma.
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DOI：
10.1186/1471-2407-4-5
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发表时间：
2004-02-06
期刊：
影响因子：
3.8
通讯作者：
Murty VV
中科院分区：
文献类型：
--
作者：
Rao PH;Arias-Pulido H;Lu XY;Harris CP;Vargas H;Zhang FF;Narayan G;Schneider A;Terry MB;Murty VV
宫颈癌是全世界女性中第二常见的癌症。放化疗相结合是晚期癌症的治疗选择。预后很差，根据疾病的不同阶段，五年存活率约为20%-65%。因此，基因特征对于了解宫颈癌的生物学和临床异质性是至关重要的。我们使用全基因组筛查方法-比较基因组杂交(CGH)来确定77例宫颈癌患者DNA拷贝数的变化。我们应用分类分析和生存分析来分析染色体改变是否与临床病理特征和患者生存有关。CGH分析显示2q33-q37缺失(57.1%)、3q增加(54.5%)和染色体扩增(20.77%)是频繁的遗传改变。共检测到15个染色体扩增位点，包括1p31、2q32、7q22、8q21.2-q24、9p22、10q21、10q24、11q13、11q21、12q15、14q12、17p11.2、17q22、18p11.2和19q13.1。11q13、11q21和19q13.1有重复扩增位点。我们进一步评估了CC患者的基因组改变对预后的意义，我们没有发现与一些临床或组织学参数有任何相关性。携带HPV18的肿瘤比携带HPV16的肿瘤表现出更高的基因组不稳定性。本研究证实2q33-q37缺失、3q获得和染色体扩增是侵袭性CC的特征性变化。这些基因突变有助于发现位于2q33-q37的新的抑癌基因(S)和位于染色体扩增部位的癌基因。利用目前的基因组技术对这些染色体变化进行分子表征将为CC的生物学和临床行为提供新的见解。
Carcinoma of uterine cervix is the second most common cancers among women worldwide. Combined radiation and chemotherapy is the choice of treatment for advanced stages of the disease. The prognosis is poor, with a five-year survival rate ranging from about 20–65%, depending on stage of the disease. Therefore, genetic characterization is essential for understanding the biology and clinical heterogeneity in cervical cancer (CC). We used a genome-wide screening method – comparative genomic hybridization (CGH) to identify DNA copy number changes in 77 patients with cervical cancer. We applied categorical and survival analyses to analyze whether chromosomal changes were related to clinico-pathologic characteristics and patients survival. The CGH analysis revealed a loss of 2q33-q37 (57.1%), gain of 3q (54.5%) and chromosomal amplifications (20.77%) as frequent genetic changes. A total of 15 amplified chromosomal sites were detected in 16 cases that include 1p31, 2q32, 7q22, 8q21.2-q24, 9p22, 10q21, 10q24, 11q13, 11q21, 12q15, 14q12, 17p11.2, 17q22, 18p11.2, and 19q13.1. Recurrent amplified sites were noted at 11q13, 11q21, and 19q13.1. The genomic alterations were further evaluated for prognostic significance in CC patients, and we did not find any correlation with a number of clinical or histological parameters. The tumors harboring HPV18 exhibited higher genomic instability compared to tumors with HPV 16. This study demonstrated that 2q33-q37 deletions, 3q gains and chromosomal amplifications as characteristic changes in invasive CC. These genetic alterations will aid in the identification of novel tumor suppressor gene(s) at 2q33-q37 and oncogenes at amplified chromosomal sites. Molecular characterization of these chromosomal changes utilizing the current genomic technologies will provide new insights into the biology and clinical behavior of CC.
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影响因子：
8
作者：
Narayan, G;Pulido, HA;Murty, VVVS
通讯作者：
Murty, VVVS
DOI：
10.1073/pnas.95.25.14717
发表时间：
1998-12-08
影响因子：
11.1
作者：
Pennica, D;Swanson, TA;Levine, AJ
通讯作者：
Levine, AJ
影响因子：
37.3
作者：
Narayan G;Arias-Pulido H;Koul S;Vargas H;Zhang FF;Villella J;Schneider A;Terry MB;Mansukhani M;Murty VV
通讯作者：
Murty VV
影响因子：
8
作者：
Ma, YY;Wei, SJ;Shen, CY
通讯作者：
Shen, CY
影响因子：
2.7
作者：
Benjamini, Y;Drai, D;Golani, I
通讯作者：
Golani, I