Sequence finishing and mapping of Drosophila melanogaster heterochromatin
Sequence finishing and mapping of Drosophila melanogaster heterochromatin
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DOI：
10.1126/science.1139816
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发表时间：
2007-06-15
期刊：
影响因子：
56.9
通讯作者：
Celniker, Susan E.
中科院分区：
文献类型：
--
作者：
Hoskins, Roger A.;Carlson, Joseph W.;Celniker, Susan E.
大多数后生动物和植物的基因组序列是不完整的，因为异染色质中存在重复的DNA。黑腹果蝇的异染色质区含有2000万个碱基（Mb）的序列，适合于作图、序列组装和整理。我们描述了使用可用的克隆资源和组装方法产生15 Mb的成品或改进的异染色质序列。我们还构建了一个细菌人工染色体为基础的物理地图，跨越13 Mb的近着丝粒异染色质和细胞遗传学地图，位置11 Mb在特定的染色体位置。我们已经接近一个完整的装配和映射的果蝇异染色质的非卫星组成部分。我们所描述的策略也适用于产生更多的信息异染色质在其他物种，包括人类。
Genome sequences for most metazoans and plants are incomplete because of the presence of repeated DNA in the heterochromatin. The heterochromatic regions of Drosophila melanogaster contain 20 million bases (Mb) of sequence amenable to mapping, sequence assembly, and finishing. We describe the generation of 15 Mb of finished or improved heterochromatic sequence with the use of available clone resources and assembly methods. We also constructed a bacterial artificial chromosome-based physical map that spans 13 Mb of the pericentromeric heterochromatin and a cytogenetic map that positions 11 Mb in specific chromosomal locations. We have approached a complete assembly and mapping of the nonsatellite component of Drosophila heterochromatin. The strategy we describe is also applicable to generating substantially more information about heterochromatin in other species, including humans.