The oxysterol and cholestenoic acid profile of mouse cerebrospinal fluid.
The oxysterol and cholestenoic acid profile of mouse cerebrospinal fluid.
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DOI：
10.1016/j.steroids.2015.02.021
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发表时间：
2015-07
期刊：
影响因子：
2.7
通讯作者：
Griffiths WJ
中科院分区：
文献类型：
--
作者：
Crick PJ;Beckers L;Baes M;Van Veldhoven PP;Wang Y;Griffiths WJ
通过LC-MS测量小鼠CSF中的氧固醇和异戊烯酸。小鼠CSF中的主要胆固醇代谢产物为7α-羟基化的异戊烯酸。CSF中的胆固醇代谢物水平比血浆低一个数量级。24 S-羟基胆固醇的7α-羟基代谢产物见于CSF而非血浆。氧化固醇和异戊烯酸是胆固醇的氧化形式，具有许多生物学功能。氧固醇在各种神经系统疾病中的可能作用使得对中枢神经系统中这些代谢物的分析特别感兴趣。在这里，我们报告的识别和定量的一组12甾醇在小鼠脑脊液（CSF）中使用液相色谱-质谱利用酶辅助衍生甾醇分析技术。我们在CSF中发现低水平的氧固醇和异戊烯酸，范围为5 pg/mL-2.6 ng/mL。在人体中发现，这些浓度比血浆中低一到两个数量级。
Measurement of oxysterols and cholestenoic acids in mouse CSF by LC–MS. Major cholesterol metabolites in mouse CSF are 7α-hydroxylated cholestenoic acids. Levels of cholesterol metabolites an order of magnitude lower in CSF than plasma. 7α-Hydroxy metabolites of 24S-hydroxycholesterol found in CSF but not plasma. Oxysterols and cholestenoic acids are oxidised forms of cholesterol with a host of biological functions. The possible roles of oxysterols in various neurological diseases makes the analysis of these metabolites in the central nervous system of particular interest. Here, we report the identification and quantification of a panel of twelve sterols in mouse cerebrospinal fluid (CSF) using liquid chromatography–mass spectrometry exploiting enzyme assisted derivatisation for sterol analysis technology. We found low levels of oxysterols and cholestenoic acids in CSF in the range of 5 pg/mL–2.6 ng/mL. As found in man, these concentrations are one to two orders of magnitude lower than in plasma.