Discerning the effects of photoinhibition and photoprotection on the rate of oxygen evolution in Arabidopsis leaves
Discerning the effects of photoinhibition and photoprotection on the rate of oxygen evolution in Arabidopsis leaves
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DOI：
10.1016/j.jphotobiol.2015.09.010
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发表时间：
2015-11-01
影响因子：
5.4
通讯作者：
Ruban, Alexander V.
中科院分区：
文献类型：
--
作者：
Giovagnetti, Vasco;Ruban, Alexander V.
高等植物具有一套相互关联的过程来调节光捕获。叶绿素a荧光（NPQ）的非光化学猝灭是保护光系统（PS）II免受过量光能吸收的最快过程。然而，PSII反应中心（RCIs）的损伤往往是不可避免的，这种现象称为光抑制。NPQ和光抑制均破坏PSII量子产率（Phi（PSII））。最近，我们设计了一种基于荧光的方法，该方法使用光照后在黑暗中测量的光化学猝灭系数（qPd）来评估RCIls的完整性。该程序能够将Phi（PSII）表示为NPQ和qPd的函数（f），Phi（PSII）= f（NPQ qPd），从而允许有效地辨别保护性NPQ和光抑制对电子传递效率的影响。在这项研究中，我们解决了qPd和Phi（PSII）的拟南芥完整叶片光合放氧测量之间的关系。我们发现，qPd和Phi（PSII）的氧释放（以及Fv/Fm）之间的线性相关性。这与qPd反映光抑制的开始有关。这些结果进一步证明了qPd参数的有效性和基本理论，在定量评估PSII效率单独使用这种有效的和简单的荧光技术。(C)2015 Elsevier B. V.版权所有。
Higher plants possess a set of interconnected processes to regulate light harvesting. Non-photochemical quenching of chlorophyll a fluorescence (NPQ) is the fastest process activated to protect the photosystem (PS) II from the absorption of excess light energy. However, damage of PSII reaction centers (RCIls) is often inevitable, a phenomenon known as photoinhibition. Both NPQand photoinhibition undermine PSII quantum yield (Phi(PSII)). Recently, we devised a fluorescence-based methodology that uses the coefficient of photochemical quenching measured in the dark following illumination (qPd) to assess the intactness of RCIls. This procedure enables to express Phi(PSII) as a function (f) of NPQand qPd, Phi(PSII) = f(NPQqPd), thus allowing to efficiently discern between the effects of protective NPQ and photoinhibition upon the efficiency of electron transport. In this study, we addressed the relationship between qPd and Phi(PSII) measured by photosynthetic oxygen evolution in intact leaves of Arabidopsis. We found a linear correlation between qPd and Phi(PSII) of oxygen evolution (as well as Fv/Fm). This relates to the fact that qPd reflects the onset of photoinhibition. These results further demonstrate the validity of the qPd parameter and underlying theory in quantitatively assessing PSII efficiency solely by using this effective and simple fluorescence technique. (C) 2015 Elsevier B.V. All rights reserved.