Fabrication of a near-infrared fluorescence-emitting SiO2-AuZnFeSeS quantum dots-molecularly imprinted polymer nanocomposite for the ultrasensitive fluorescence detection of levamisole
Fabrication of a near-infrared fluorescence-emitting SiO2-AuZnFeSeS quantum dots-molecularly imprinted polymer nanocomposite for the ultrasensitive fluorescence detection of levamisole
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DOI：
10.1016/j.colsurfa.2022.129013
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发表时间：
2022-04-18
影响因子：
5.2
通讯作者：
Daeid, Niamh Nic
中科院分区：
文献类型：
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作者：
Adegoke, Oluwasesan;Zolotovskaya, Svetlana;Daeid, Niamh Nic
左旋咪唑是一种驱虫药，通常用作非法可卡因样品中的切割剂，已被列为危险物质。在这项工作中，我们报告了使用 AuZnFeSeS 合金量子点（QD）-分子印迹聚合物（MIP）纳米复合材料制造新型左旋咪唑荧光传感器。首先，通过金属前驱体、表面活性剂和有机配体的有机金属热注射热解合成了有机相 AuZnFeSeS QD。然后将二氧化硅 (SiO2) 封装在量子点表面，使其紧凑、稳定且具有生物相容性。通过自由基聚合方法，以左旋咪唑为模板的坚固的 MIP 壳被涂覆在 SiO2-AuZnFeSeS QDs 表面周围，形成 AuZnFeSeS QDs@MIP 核/壳纳米复合材料。用 MIP 壳钝化 SiO2-AuZnFeSeS QDs 核表面导致辐射激子复合过程，从而增强 QDs 荧光。有趣的是，SiO2-AuZnFeSeS QD 的特征是棒状和球形颗粒形态。在最佳条件下，AuZnFeSeS QDs@MIP 纳米复合材料的荧光在左旋咪唑存在下被猝灭，并且对其他测试药物具有选择性。在 0.5 - 100 μM 的浓度范围内实现定量检测，检测限为 0.05 μM (47.4 nM)。 AuZnFeSeS QDs@MIP 优于 AuZnFeSeS QDs@NIP（非模板化材料）的灵敏度证明了 MIP-左旋咪唑亲和力的验证。在含有可卡因的混合药物样品中成功检测到左旋咪唑，分析回收率令人满意。这项工作是首次报道使用无镉五元合金QDs@MIP纳米复合荧光检测系统来检测左旋咪唑。
Levamisole is an anthelminthic drug that is usually used as a cutting agent to lace illicit cocaine samples and has been classified as a dangerous substance. In this work, we report on the fabrication of a novel fluorescence sensor for levamisole using AuZnFeSeS alloyed quantum dots (QDs)-molecularly imprinted polymer (MIP) nano composite. Firstly, organic-phased AuZnFeSeS QDs were synthesized via the organometallic hot-injection pyrolysis of metal precursors, surfactants, and organic ligands. Silica (SiO2) was then encapsulated on the QDs surface to render it compact, stable, and biocompatible. Via a free radical polymerization approach, a robust MIP shell templated with levamisole was coated around the SiO2-AuZnFeSeS QDs surface to form a AuZnFeSeS QDs@MIP core/shell nanocomposite. Passivation of the SiO2-AuZnFeSeS QDs core surface with the MIP shell led to radiative exciton recombination process, whereby the QDs fluorescence was enhanced. Interestingly, the SiO2- AuZnFeSeS QDs was characterized by rod-shaped and spherical-shaped particle morphology. Under optimum conditions, the fluorescence of the AuZnFeSeS QDs@MIP nanocomposite was quenched in the presence of levamisole and was selective against other tested drugs. Quantitative detection was achieved in the concentration range of 0.5 - 100 mu M with a limit of detection of 0.05 mu M (47.4 nM). Validation of the MIP-levamisole affinity was demonstrated by the superior sensitivity of the AuZnFeSeS QDs@MIP over the AuZnFeSeS QDs@NIP (nontemplated material). Levamisole was successfully detected in a mixed drug sample containing cocaine with satisfactory analytical recoveries. This work is the first reported use of a cadmium-free quintenary alloyed QDs@MIP nanocomposite fluorescence detection system for levamisole.