Reproducibility of nerve fiber thickness, macular thickness, and optic nerve head measurements using StratusOCT
Reproducibility of nerve fiber thickness, macular thickness, and optic nerve head measurements using StratusOCT
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DOI：
10.1167/iovs.03-0514
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发表时间：
2004-06-01
影响因子：
4.4
通讯作者：
Fujimoto, JG
中科院分区：
文献类型：
--
作者：
Paunescu, LA;Schuman, JS;Fujimoto, JG
目的。研究了第三代商用光学相干断层扫描仪 OCT-3（StratusOCT，软件版本 A2，Carl Zeiss Meditec Inc.，都柏林，加利福尼亚州）的测量再现性。研究正常眼的神经纤维层（NFL）厚度、黄斑厚度图和视神经乳头（ONH）参数。方法。十名正常受试者每天成像六次（扩张前三次，扩张后三次），并在不同的三天重复该系列。在扫描的 3 天中，瞳孔扩张前的扫描顺序是随机的。瞳孔扩张后，在 3 天的扫描中每天进行随机扫描。每个系列分别进行标准密度（每个黄斑和 ONH 图像 128 个 A 扫描，每个 NFL 图像 256 个 A 扫描）和高密度（所有三种扫描类型每个图像 512 个 A 扫描）扫描。结果。平均黄斑厚度为 235 +/- 9.8 μm。 A 扫描密度（或图像采集速度）对平均黄斑厚度、黄斑体积和黄斑图的一些扇区的可重复性具有统计显着性影响（P < 0.05）。对于任何黄斑参数均未发现显着的扩张效应。散瞳高密度扫描发现黄斑扫描的最佳组内相关系数（ICC；94%），访问间 SD 为 2.4 μm，访问间 SD 为 2.2 μm。标准扫描的平均 NFL 厚度为 98 +/- 9 μm。 NFL 的再现性显示出不同的结果，并且某些参数的扫描密度和扩张之间存在交互作用。对于大多数 NFL 参数，扩张标准密度扫描的重现性更好。扩张标准扫描的平均 NFL 厚度 ICC 为 79%，访视间 SD 为 2.5 μm，访视内 SD 为 1.6 μm。对于 ONH 分析，几乎所有参数的扩张标准密度扫描的重现性都更好，除了椎间盘面积、水平积分边缘体积和垂直积分边缘面积（在扩张之前更好）之外。杯盘比具有最佳重现性（ICC = 97%，访视间 SD 为 0.04 μm，访视内 SD 为 0.02 μm）。结论。 StratusOCT 证明了 NFL 厚度、黄斑厚度和视神经乳头参数的可重复测量。 NFL 和 ONH 参数的散瞳标准扫描以及黄斑参数的散瞳高密度扫描具有最佳重现性。
PURPOSE. The measurement reproducibility of the third generation of commercial optical coherence tomography, OCT-3 (StratusOCT, software ver. A2, Carl Zeiss Meditec Inc., Dublin, CA) was investigated. The nerve fiber layer (NFL) thickness, macula thickness map, and optic nerve head (ONH) parameters in normal eyes were studied.METHODS. Ten normal subjects were imaged six times (three before and three after dilation) per day, and the series was repeated on three different days. The order of the scans before pupil dilation was randomized in each of the 3 days of scanning. After pupil dilation, the scans were also randomized in each of the 3 days of scanning. Each series was performed separately for standard-density (128 A-scans per macular and ONH image and 256 A-scans per NFL image) and high-density (512 A-scans per image for all three scan types) scanning.RESULTS. The mean macular thickness was 235 +/- 9.8 mum. A-scan density (or image acquisition speed) had a statistically significant effect (P < 0.05) on the reproducibility of the mean macular thickness, macular volume, and a few sectors of the macular map. No significant dilation effect was found for any of the macular parameters. The best intraclass correlation coefficient (ICC; 94%) for macular scans was found for dilated high-density scanning, with an intervisit SD of 2.4 mu m and an intravisit SD of 2.2 mu m. The mean NFL thickness for standard scanning was 98 +/- 9 mu m. NFL reproducibility showed mixed results and had interactions between scan density and dilation for some parameters. For most of the NFL parameters, reproducibility was better with dilated standard-density scanning. The mean NFL thickness ICC for dilated standard scanning was 79%, with an intervisit SD of 2.5 mu m and an intravisit SD of 1.6 mu m. For the ONH analysis, the reproducibility was better for dilated standard-density scanning for almost all the parameters, except for disc area, horizontal integrated rim volume, and vertical integrated rim area, which were better before dilation. The best reproducibility was found for cup-to-disc ratio (ICC = 97%, with intervisit SD of 0.04 mu m and intravisit SD of 0.02 mu m).CONCLUSIONS. StratusOCT demonstrated reproducible measurements of NFL thickness, macular thickness, and optic nerve head parameters. The best reproducibility was found for dilated standard scanning for NFL and ONH parameters and for dilated high-density scanning for macular parameters.