Preventive effect of Lactobacillus fermentum Lee on activated carbon-induced constipation in mice
Preventive effect of Lactobacillus fermentum Lee on activated carbon-induced constipation in mice
复制标题
DOI：
10.3892/etm.2014.2064
复制
发表时间：
2015-01-01
影响因子：
2.7
通讯作者：
Liu, Zhenhu
中科院分区：
文献类型：
--
作者：
Qian, Yu;Suo, Huayi;Liu, Zhenhu
本研究旨在探讨发酵乳杆菌（LF-Lee）对活性炭诱导的ICR小鼠便秘的影响。ICR小鼠口服乳酸菌9天。观察各组大鼠体重、饮水量、排便情况、胃肠转运时间、排便时间以及血清胃动素（MTL）、胃泌素（Gas）、内皮素（ET）、生长抑素（SS）、乙酰胆碱酯酶（AChE）、P物质（SP）和血管活性肠肽（VIP）水平。给予比沙可啶（一种泻药）作为阳性对照。正常、对照、比沙可啶（100 mg/kg，经口给药）、保加利亚乳杆菌（LB）、LF-Lee低剂量（L）和LF-Lee高剂量（H）给药小鼠首次排黑便所需的时间分别为90、218、117、180、161和151 min。在摄入LB、LF-Lee（L）或LF-Lee（H）或经口给予比沙可啶后，GI转运分别降低至正常小鼠中转运的55.2%、65.8%、73.1%和94.6%。LF-Lee治疗组小鼠血清MTL、Gas、ET、AChE、SP、VIP水平显著高于对照组，SS水平显著低于对照组（P
The aim of this study was to investigate the effects of Lactobacillus fermentum Lee (LF-Lee) on activated carbon-induced constipation in ICR mice. ICR mice were orally administered lactic acid bacteria for nine days. Body weight, dietary and water intake, defecation status, gastrointestinal (GI) transit and defecation time, as well as levels of motilin (MTL), gastrin (Gas), endothelin (ET), somatostatin (SS), acetylcholinesterase (AChE), substance P (SP) and vasoactive intestinal peptide (VIP) in serum were measured to evaluate the preventive effects of LF-Lee on constipation. Bisacodyl, a laxative drug, was administered as a positive control. The time taken until the first defecation of a black stool for normal, control, bisacodyl- (100 mg/kg, oral administration), Lactobacillus bulgaricus (LB)-, LF-Lee low dose (L)- and LF-Lee high dose (H)-treated mice was 90, 218, 117, 180, 161 and 151 min, respectively. Following the consumption of LB, LF-Lee (L) or LF-Lee (H), or the oral administration of bisacodyl, the GI transit was reduced to 55.2, 65.8, 73.1 and 94.6%, respectively, of the transit in normal mice. The serum levels of MTL, Gas, ET, AChE, SP and VIP were significantly increased and those of SS were reduced in the mice treated with LF-Lee compared with those in the untreated control mice (P