Ultrastructure of the intercalated body, a novel organelle associated with fluid forming cells in the organ of Corti.
Ultrastructure of the intercalated body, a novel organelle associated with fluid forming cells in the organ of Corti.
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闰体的超微结构，一种与柯蒂氏器中液体形成细胞相关的新型细胞器。
DOI：
10.1002/jemt.1060150309
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Scott,GL
中科院分区：
文献类型：
--
作者：
Sobkowicz,HM;Holy,J;Scott,GL
闰体是在小鼠Corti器内、外螺旋沟细胞中新发现的一种细胞器。在14天及以上完整小鼠的耳蜗中以及相应年龄培养物中的器官中发现了该细胞器。细胞器由一堆相互连接的内质网池和膜结合的小棒组成，小棒插入池之间并平行于池运行。池膜主要是光滑的，但有些可以显示核糖体。大多数小棒长1 ~ 2 μm，宽约0.1 μm，含有电子致密物质。线粒体通常与细胞器相关或并入细胞器中。一些电子显微照片表明，小杆可能来自修改线粒体。我们的印象是，细胞器的形成始于池和线粒体的并置。脑池相关的线粒体似乎收缩、伸长并失去其内膜。在这两个完整的动物和文化中，内，外螺旋沟的细胞显示微绒毛，顶端交界复合体，横向细胞间隙含有交错的细胞过程，并似乎参与流体的形成。此外，在培养中，内外螺旋沟的细胞以及在生长区增殖的一些细胞参与流体形成，产生大的流体袋。所有这些细胞通常含有插入体。这是可能的，在完整的动物，在文化中，插入体可能发挥作用，在流体调节附近的毛细胞。
The intercalated body is a newly discovered organelle in the inner and outer spiral sulcus cells of the mouse organ of Corti. The organelle was found in the cochleas of 14‐day and older intact mice and in organs in culture of corresponding ages. The organelle consists of a stack of interconnected cisternae of endoplasmic reticulum and of membrane bound rodlets that are intercalated between, and run parallel to, the cisternae. The cisternal membranes are predominantly smooth, but some may display ribosomes. Most rodlets are from 1 to 2 μm long, about 0.1 μm wide, and contain electron dense material. Mitochondria are commonly associated with or incorporated into the organelle. Some electron micrographs suggest that the rodlets may originate from modified mitochondria. It is our impression that the formation of the organelle begins with the apposition of cisternae and mitochondria. Cisternal‐associated mitochondria appear to constrict, elongate, and lose their inner membranes. In both the intact animal and in culture, the cells of the inner and outer spiral sulci display microvilli, apical junctional complexes, lateral intercellular spaces containing interdigitating cell processes, and appear to be involved in fluid formation. Moreover, in culture, the cells of inner and outer spiral sulci as well as some cells proliferating in the outgrowth zone participate in fluid formation, producing large fluid pockets. All these cells commonly contain intercalated bodies. It is possible that in the intact animal, as in culture, intercalated bodies may play a role in fluid regulation in the immediate vicinity of the hair cells.