Oxygen regulation and limitation to cellular respiration in mouse skeletal muscle in vivo
Oxygen regulation and limitation to cellular respiration in mouse skeletal muscle in vivo
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DOI：
10.1152/ajpheart.00192.2003
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发表时间：
2003-11-01
影响因子：
4.8
通讯作者：
Schenkman, KA
中科院分区：
文献类型：
--
作者：
Marcinek, DJ;Ciesielski, WA;Schenkman, KA
在骨骼肌中，细胞内PO 2可降至2 - 3 mmHg。本研究测试了在此范围的氧张力下，氧是否通过磷酸化电位和细胞内PO 2之间的直接耦合来调节细胞呼吸。氧也可以作为细胞呼吸中的简单底物，在大多数生理范围内接近饱和水平。使用Swiss-Webster小鼠缺血后肢肌肉中血红蛋白和肌红蛋白饱和度的下降，使用一种新的光学光谱方法测量组织耗氧量（（M）/点O-2）和细胞内PO 2。P-31磁共振光谱测定产生磷酸肌酸浓度（[PCr]）和pH值在相同的肌肉体积。细胞内PO 2下降至
In skeletal muscle, intracellular PO2 can fall to as low as 2 - 3 mmHg. This study tested whether oxygen regulates cellular respiration in this range of oxygen tensions through direct coupling between phosphorylation potential and intracellular PO2. Oxygen may also behave as a simple substrate in cellular respiration that is near saturating levels over most of the physiological range. A novel optical spectroscopic method was used to measure tissue oxygen consumption ((M) over dot O-2) and intracellular PO2 using the decline in hemoglobin and myoglobin saturation in the ischemic hindlimb muscle of Swiss-Webster mice. P-31 magnetic resonance spectroscopic determinations yielded phosphocreatine concentration ([PCr]) and pH in the same muscle volume. Intracellular PO2 fell to