GLUCOSE INDUCES CAMP-INDEPENDENT GROWTH-RELATED CHANGES IN STATIONARY-PHASE CELLS OF SACCHAROMYCES-CEREVISIAE
GLUCOSE INDUCES CAMP-INDEPENDENT GROWTH-RELATED CHANGES IN STATIONARY-PHASE CELLS OF SACCHAROMYCES-CEREVISIAE
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DOI：
10.1073/pnas.88.13.5724
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发表时间：
1991-07-01
影响因子：
11.1
通讯作者：
SNYDER, M
中科院分区：
文献类型：
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作者：
GRANOT, D;SNYDER, M
营养素在决定启动新的细胞周期中起着关键作用。向停滞的细胞如静止期细胞和孢子中加入营养物可诱导它们开始生长。我们分析了诱导酵母早期细胞事件所需的营养物质。当静止期细胞或孢子在仅存在葡萄糖的情况下孵育时，诱导有丝分裂生长细胞的形态和生理特征变化，并且在不存在支持生长的额外营养物的情况下，细胞迅速失去活力。在葡萄糖存在下预孵育静止期细胞减少了随后加入丰富培养基时达到芽出现所需的时间。这些过程是由D-葡萄糖特异性诱导的，而不是由其他组分如氮源或L-葡萄糖诱导的。葡萄糖诱导的事件是独立的腺苷酸环化酶途径，因为无论是在腺苷酸环化酶基因（CDC 35）或其调节（CDC 25）的温度敏感性突变的菌株进行葡萄糖诱导的细胞变化时，在限制性温度下孵育。我们认为，葡萄糖触发事件在一个新的有丝分裂细胞周期的诱导，这些事件之前的腺苷酸环化酶途径或在一个替代途径。
Nutrients play a critical role in the decision to initiate a new cell cycle. Addition of nutrients to arrested cells such as stationary-phase cells and spores induces them to begin growth. We have analyzed the nutrients required to induce early cellular events in yeast. When stationary-phase cells or spores are incubated in the presence of only glucose, morphological and physiological changes characteristic of mitotically growing cells are induced and, in the absence of additional nutrients to support growth, the cells rapidly lose viability. Preincubation of stationary-phase cells in the presence of glucose decreases the time required to reach bud emergence upon the subsequent addition of rich medium. These processes are specifically induced by D-glucose and not by other components such as nitrogen source or L-glucose. The glucose-induced events are independent of the adenylate cyclase pathway, since strains with a temperature-sensitive mutation in either the adenylate cyclase gene (CDC35) or its regulator (CDC25) undergo glucose-induced cellular changes when incubated at the restrictive temperature. We suggest that glucose triggers events in the induction of a new mitotic cell cycle and that these events are either prior to the adenylate cyclase pathway or are in an alternative pathway.