Ontogeny of the epidermal growth factor receptor during development of the fetal bovine mesonephros and associated organs of the urogenital tract.
Ontogeny of the epidermal growth factor receptor during development of the fetal bovine mesonephros and associated organs of the urogenital tract.
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胎牛中肾和泌尿生殖道相关器官发育过程中表皮生长因子受体的个体发育。
DOI：
10.1095/biolreprod48.6.1395
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发表时间：
1993
影响因子：
3.6
通讯作者：
Gorski,J
中科院分区：
文献类型：
--
作者：
Winters,TA;Febres,FG;Fulgham,DL;Bertics,PJ;Duello,TM;Gorski,J
为研究胎牛中肾及其泌尿生殖系统中的表皮生长因子(EGF)受体，建立了原代细胞培养系统。放射受体分析表明，早在第37天，中肾细胞和来自胎儿生殖管、性腺和后肾的细胞就与EGF结合--所有这些都是中肾衍生物。免疫细胞化学研究表明，EGF受体定位于这些泌尿生殖器官的导管和肾小管上皮。EGF诱导牛中肾细胞DNA合成和酪氨酸磷酸化，提示在这些细胞中检测到的EGF受体具有功能。此外，转化生长因子α被认为是EGF受体的胎儿配体，被发现特异性地竞争EGF与受体的结合，并以类似于EGF的方式诱导DNA合成。雌激素不能调节EGF受体，也不能与牛中肾细胞特异性结合。与小鼠相比，牛的雌激素受体的发育明显晚于EGF受体的发育，小鼠的雌激素受体和EGF受体都是在生殖道发育的非常早期阶段观察到的。
A primary cell culture system was developed to study the epidermal growth factor (EGF) receptor in the fetal bovine mesonephros and its urogenital derivatives. Radioreceptor assays demonstrated EGF binding as early as Day 37 in mesonephric cells and in cells derived from the fetal reproductive ducts, gonads, and metanephros—all mesonephric derivatives. Immunocytochemical studies revealed that EGF receptors were localized in the ductal and tubular epithelium of these urogenital organs. EGF induced DNA synthesis and tyrosine phosphorylation in the bovine mesonephric cells, suggesting that EGF receptors detected in these cells were functional. In addition, transforming growth factor alpha, the putative fetal ligand for the EGF receptor, was found to specifically compete for EGF binding to the receptor, as well as to induce DNA synthesis in a manner similar to that of EGF. Estrogen did not regulate EGF receptors or specifically bind to bovine mesonephric cells. The development of estrogen receptors in the bovine species occurs markedly later than that of the EGF receptor, in contrast to the mouse, where both receptors are observed at very early stages of reproductive tract development.