Relationships between NADPH diaphorase staining and neuronal, endothelial, and inducible nitric oxide synthase and cytochrome P450 reductase immunoreactivities in guinea-pig tissues.
Relationships between NADPH diaphorase staining and neuronal, endothelial, and inducible nitric oxide synthase and cytochrome P450 reductase immunoreactivities in guinea-pig tissues.
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豚鼠组织中 NADPH 心肌黄酶染色与神经元、内皮、诱导型一氧化氮合酶和细胞色素 P450 还原酶免疫反应性之间的关系。
DOI：
10.1007/s004180050085
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Tracey,WR
中科院分区：
文献类型：
--
作者：
Young,HM;O'Brien,AJ;Furness,JB;Ciampoli,D;Hardwick,JP;McCabe,TJ;Narayanasami,R;Masters,BS;Tracey,WR
NADPH黄递酶染色的存在进行了比较与免疫组化定位的四个NADPH依赖性酶-神经元（I型），诱导型（II型），和内皮细胞（III型）一氧化氮合酶（NOS）和细胞色素P450还原酶。对NADPH依赖性酶免疫反应的细胞类型也被染色为NADPH黄递酶，表明内皮和神经元NOS和细胞色素P450还原酶在甲醛固定的组织中均显示NADPH黄递酶活性。然而，在某些组织中，NADPH黄递酶染色的存在与我们检查的任何NADPH依赖性酶的存在并不一致。在血管内皮细胞中，用NADPH黄递酶组织化学观察到的点状染色模式与使用内皮NOS抗体的免疫组织化学观察到的相同。在肠和胰腺神经元和骨骼肌中，NADPH黄递酶染色的存在与神经元NOS的存在相关。在肝脏、皮肤的皮脂腺、纤毛上皮和气管的浆膜下腺、肾上腺皮质的肾小球、泪腺和唾液腺的上皮细胞中的细胞亚群中，NADPH黄递酶染色的存在与细胞色素P450还原酶免疫反应性的存在相一致。在肾小管上皮细胞和肾上腺皮质束状核和网状核中，观察到NADPH黄递酶染色，与任何酶的存在不一致。在任何组织中均未观察到诱导型NOS。因此，虽然对神经元和内皮NOS表现出免疫反应性的组织也对NADPH黄递酶活性呈阳性染色，但NADPH黄递酶染色的存在并不能可靠地或特异性地指示一种或多种NOS同种型的存在。
The presence of NADPH diaphorase staining was compared with the immunohistochemical localization of four NADPH-dependent enzymes – neuronal (type I), inducible (type II), and endothelial (type III) nitric oxide synthase (NOS) and cytochrome P450 reductase. Cell types that were immunoreactive for the NADPH-dependent enzymes were also stained for NADPH diaphorase, suggesting that endothelial and neuronal NOS and cytochrome P450 reductase all show NADPH diaphorase activity in formaldehyde-fixed tissue. However, in some tissues, the presence of NADPH diaphorase staining did not coincide with the presence of any of the NADPH-dependent enzymes we examined. In vascular endothelial cells, the punctate pattern of staining observed with NADPH diaphorase histochemistry was identical to that seen following immunohistochemistry using antibodies to endothelial NOS. In enteric and pancreatic neurons and in skeletal muscle, the presence of NADPH diaphorase staining correlated with the presence of neuronal NOS. In the liver, sebaceous glands of the skin, ciliated epithelium, and a subpopulation of the cells in the subserosal glands of the trachea, zona glomerulosa of the adrenal cortex, and epithelial cells of the lacrimal and salivary glands, the presence of NADPH diaphorase staining coincided with the presence of cytochrome P450 reductase immunoreactivity. In epithelial cells of the renal tubules and zona fasciculata and zona reticularis of the adrenal cortex, NADPH diaphorase staining was observed that did not coincide with the presence of any of the enzymes. Inducible NOS was not observed in any tissue. Thus, while tissues that demonstrate immunoreactivity for neuronal and endothelial NOS also stain positively for NADPH diaphorase activity, the presence of NADPH diaphorase staining does not reliably or specifically indicate the presence of one or more NOS isoforms.