Multiple elements are required for expression of an intermediate filament gene.
Multiple elements are required for expression of an intermediate filament gene.
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DOI：
10.1093/nar/16.16.8057
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发表时间：
1988-08
影响因子：
14.9
通讯作者：
C. M. Sax;F. Farrell;J. A. Tobian;Z. Zehner
中科院分区：
文献类型：
--
作者：
C. M. Sax;F. Farrell;J. A. Tobian;Z. Zehner
The expression of vimentin is unique within the intermediate filament multigene family. It is the only member which deviates from its usual tissue-specific expression pattern and whose 5'-flanking region contains multiple GC boxes, the binding site for Sp1. The activity of vimentin 5'-end:CAT fusions has been compared in cells where vimentin is highly expressed (mouse L cells) or not expressed at all (MH1C1). In addition, CAT activity has been examined by microinjection into Xenopus oocytes. Both in vivo expression and in vitro binding studies implicate Sp1 as a general regulatory factor in vimentin gene expression. Increased expression of 5'-end:CAT fusions in mouse L cells suggests that a fibroblast-specific enhancer element resides in the region -321 to -160. Low transcriptional activity in MH1C1 cells may be due to either the lack of this positive transcription factor(s) or the presence of a repressor element. Here, we demonstrate that the unique and complex pattern of vimentin gene expression is controlled by multiple cis-acting elements.
