Morphological classification of the rat lateral cerebellar nuclear neurons by principal component analysis
Morphological classification of the rat lateral cerebellar nuclear neurons by principal component analysis
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主成分分析法对大鼠小脑外侧核神经元的形态学分类
DOI：
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发表时间：
2003
期刊：
影响因子：
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通讯作者：
P. Thier
中科院分区：
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作者：
F. Sultan;U. Czubayko;P. Thier
小脑深部核（DCN）是小脑向大脑其他部分传递信息的主要结构。尽管DCN的连通性已经得到了很好的研究，但对于DCN输入和输出之间的接口（神经元的体细胞和树突）知之甚少。因此，我们决定通过多变量方法（主成分分析；PCA）来分析神经元的体细胞和树突形态，以确定可能与核微电路中不同位置相关的形态群。PCA是基于大鼠侧DCN细胞内染色的神经元和描述神经元形态的19个参数。PCA产生了两个主成分，占方差的46%。第一个成分与体细胞大小有关，将大部分神经元（I型）与一小部分神经元（II型）分开。第二个成分与具有更多初级树突和多极外观的较大细胞呈负相关（Ia型），与具有不对称树突场和簇状树突的较小神经元呈正相关（Ib型）。我们的Ib型神经元中小体体的优势表明这些神经元可能对应于下橄榄投射神经元。总之，我们的结果与之前的分类一致，即将投射神经元（I型）与局部神经元（II型）区分开来；此外，我们的研究结果指出了迄今为止尚未描述的投射神经元树突形态差异。后者对于理解哺乳动物小脑外侧核的系统发育变化可能很重要。中国生物医学工程学报，2004,25(5):559 - 559。©2002 Wiley‐Liss, Inc。
The deep cerebellar nuclei (DCN) constitute the major structures by which the cerebellum forwards its output to the rest of the brain. Although the connectivity of the DCN has been well studied, little is known about the interface—the neurons' soma and dendrites—between the DCN's inputs and outputs. We therefore decided to analyze the neurons' somatic and dendritic morphology by applying a multivariate approach (principal component analysis; PCA), in order to define morphological groups possibly related to distinct positions in the nuclear microcircuitry. The PCA was based on intracellularly stained neurons from the rat's lateral DCN and on 19 parameters that described the neurons' morphology. The PCA yielded two principal components that accounted for 46% of the variance. The first component, correlated with soma size, separated the majority of neurons (type I) from a population of small neurons (type II). The second component showed negative correlation with larger cells with more numerous primary dendrites and a more multipolar appearance (type Ia) and positive correlation with smaller neurons with asymmetric dendritic fields and tufted dendrites (type Ib). The preponderance of small somata in our type Ib neurons suggests that these neurons probably correspond to the inferior olive projection neurons. In summary, our results are in agreement with previous classifications, which distinguished projection neurons (type I) from local neurons (type II); furthermore, our results point to a hitherto undescribed dendritic morphological difference in the projection neurons. The latter may be important for understanding the phylogenetic changes seen in the mammalian lateral cerebellar nucleus. J. Comp. Neurol. 455:139–155, 2003. © 2002 Wiley‐Liss, Inc.