Expression of epidermal growth factors, erbBs, in the nasal mucosa of patients with chronic hypertrophic rhinitis.
Expression of epidermal growth factors, erbBs, in the nasal mucosa of patients with chronic hypertrophic rhinitis.
复制标题
慢性肥厚性鼻炎患者鼻粘膜中表皮生长因子 erbB 的表达。
DOI：
10.1159/000335583
复制
发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Nguyen KH
中科院分区：
文献类型：
--
作者：
岡秀樹;都築建三;児島雄介;竹林宏記;阪上雅史.;福島久毅;Nguyen KH
目的：研究慢性肥厚性鼻炎患者鼻黏膜中erbBs的表达。方法：收集12例变应性和非变应性慢性肥厚性鼻炎患者的下鼻甲，行下鼻甲切除术。术前进行外周血细胞分类计数和过敏试验。采用荧光免疫组化和实时定量转录聚合酶链反应（qRT-PCR）检测erbB 1、2、3和4的表达。免疫组织化学荧光定量测量使用图像分析软件。结果：erbB 1-4型在鼻粘膜上皮细胞和鼻腺细胞中均有表达。erbB 1免疫反应性强，erbB 2和3为中等，erbB 4为弱。这些发现与qRT-PCR的结果一致。外周血嗜酸性粒细胞的百分比显着相关的嗜酸性粒细胞计数在鼻粘膜和免疫反应erbB 1在鼻gland.Conclusions：这些结果表明嗜酸性粒细胞在调节erbB 1，从而在调节慢性肥厚性鼻炎粘膜肥大可能发挥的作用。
Purpose:To investigate the expression of erbBs in the nasal mucosa of patients with chronic hypertrophic rhinitis.Procedures:Inferior turbinates were collected from 12 turbinectomized patients with allergic and nonallergic chronic hypertrophic rhinitis. Differential cell counts in the peripheral blood and allergy tests were conducted before surgery. The expressions of erbB1, 2, 3 and 4 were examined by fluorescence immunohistochemistry and by quantitative real-time transcription polymerase chain reaction (qRT-PCR). Immunohistochemical fluorescence was quantitatively measured using image-analyzing software. Eosinophils infiltrated into the mucosa were counted in sections stained with Hansel solution.Results:Each of the erbB types 1-4 was expressed in both epithelial cells and nasal gland cells. Immunoreactivity for erbB1 was strong and that for erbB2 and 3 was moderate, while that for erbB4 was faint. These findings were consistent with the results of qRT-PCR. The percentage of peripheral blood eosinophils was significantly correlated with the eosinophil count in the nasal mucosa and with immunoreactivity for erbB1 in the nasal gland.Conclusions:These results suggest a possible role of eosinophils in regulating erbB1 and thus in regulating mucosal hypertrophy in chronic hypertrophic rhinitis.