Relationships among endometrial oxytocin receptors, oxytocin-stimulated phosphoinositide hydrolysis and prostaglandin F2 alpha secretion in vitro, and plasma concentrations of ovarian steroids before and during corpus luteum regression in cyclic heifers.
Relationships among endometrial oxytocin receptors, oxytocin-stimulated phosphoinositide hydrolysis and prostaglandin F2 alpha secretion in vitro, and plasma concentrations of ovarian steroids before and during corpus luteum regression in cyclic heifers.
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子宫内膜催产素受体、催产素刺激的磷酸肌醇水解和体外前列腺素 F2 α 分泌之间的关系，以及周期性小母牛黄体退化之前和期间的卵巢类固醇血浆浓度。
DOI：
10.1095/biolreprod48.4.874
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发表时间：
1993
影响因子：
3.6
通讯作者：
Shawn S. Whiteaker
中科院分区：
文献类型：
--
作者：
M. Mirando;W. Becker;Shawn S. Whiteaker
催产素刺激子宫内膜前列腺素（PG）F2 α的分泌，并在奶牛黄体（CL）消退前后诱导子宫内膜磷酸肌醇水解。本研究调查了子宫内膜催产素受体、催产素刺激的磷酸肌醇水解和体外PGF 2 α分泌与周期性小母牛CL消退之前和期间卵巢类固醇血浆浓度之间的关系（即，发情后第13-19天）。在第13天（n = 5）、第16天（n = 5）和第19天（n = 6）从16头发情小母牛中采集生殖道。平均CL重量降低（第13、16和19天分别为4.7、4.6和3.0 +/-0.6g; p < 0.05）和血浆孕酮（第13、16和19天分别为12.2、10.5和4.3 +/- 1.5 ng/ml; p < 0.01），表明在第19天小母牛组中已开始CL消退。血浆雌二醇的平均浓度（第13、16和19天分别为3.4、3.8和5.6 +/- 1.7 pg/ml）在发情周期的不同天数之间没有显著差异（p > 0.5）。子宫内膜催产素受体的浓度在发情周期中增加（p < 0.06）（第13、16和19天分别为36、49和789 +/- 234 fmol/mg蛋白），但最大增加（p < 0.05）发生在第16和19天之间。同样，在体外用100 nM催产素诱导的子宫内膜肌醇磷酸中[3 H]肌醇的掺入也增加了（p < 0.01）发情周期（第13、16和19天，对照与催产素处理的子宫内膜的5121与5819、4529与7239和5888与68，096 +/-18，564 dpm/g组织，其中对催产素的反应性的最大增加（p < 0.01）发生在第16天和第19天之间。（250字处删节）
Oxytocin stimulates secretion of endometrial prostaglandin (PG) F2 alpha and induces endometrial phosphoinositide hydrolysis around the time of regression of the corpus luteum (CL) in cows. This study investigated the relationship between endometrial oxytocin receptors, oxytocin-stimulated phosphoinositide hydrolysis and PGF 2 alpha secretion in vitro, and plasma concentrations of ovarian steroids before and during CL regression in cyclic heifers (i.e., Days 13-19 post-estrus). Reproductive tracts were collected from 16 cyclic heifers on Days 13 (n = 5), 16 (n = 5), and 19 (n = 6). Decreases in mean CL weight (4.7, 4.6, and 3.0 +/- 0.6 g for Days 13, 16, and 19, respectively; p < 0.05) and plasma progesterone (12.2, 10.5, and 4.3 +/- 1.5 ng/ml for Days 13, 16, and 19, respectively; p < 0.01) were detected between Days 16 and 19, indicating that CL regression had been initiated in the group of Day 19 heifers. Mean concentration of plasma estradiol (3.4, 3.8, and 5.6 +/- 1.7 pg/ml for Days 13, 16, and 19, respectively) did not differ significantly (p > 0.5) among days of the estrous cycle. Concentration of endometrial oxytocin receptors increased (p < 0.06) during the estrous cycle (36, 49, and 789 +/- 234 fmol/mg protein on Days 13, 16, and 19, respectively), but the greatest increase (p < 0.05) occurred between Days 16 and 19. Similarly, incorporation of [3H]inositol into endometrial inositol phosphates induced in vitro with 100 nM oxytocin also increased (p < 0.01) during the estrous cycle (5121 vs. 5819, 4529 vs. 7239, and 5888 vs 68,096 +/- 18,564 dpm/g tissue for control vs. oxytocin-treated endometrium on Days 13, 16, and 19, respectively) with the greatest increase (p < 0.01) in responsiveness to oxytocin occurring between Days 16 and 19.(ABSTRACT TRUNCATED AT 250 WORDS)
黄体酮停药后，绵羊子宫中核雌激素受体和催产素受体迅速恢复。
DOI：
10.1016/0022-4731(85)90272-9
发表时间：
1985
期刊：
Journal of steroid biochemistry
影响因子：
--
作者：
Leavitt,WW;Okulicz,WC;McCracken,JA;Schramm,W;RobidouxJr,WF
通讯作者：
RobidouxJr,WF