Separation and direct UV detection of lanthanides complexed with cupferron by capillary electrophoresis.
Separation and direct UV detection of lanthanides complexed with cupferron by capillary electrophoresis.
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通过毛细管电泳分离和直接紫外检测与铜铁酮络合的镧系元素。
DOI：
10.1016/s0021-9673(00)00589-6
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
F. Erim
中科院分区：
文献类型：
--
作者：
N. Oztekin;F. Erim
研究了以铜铁试剂（N-亚硝基-N-苯基羟胺）为紫外吸收络合剂的毛细管区带电泳分离检测镧系元素。通过使用与铜铁试剂竞争金属离子的缓冲配体来提高部分络合的带正电荷的铜铁试剂络合物的分辨率。当使用羟基异丁酸（HIBA）作为缓冲液和竞争配体时，它提供了完全分离所有14个镧系元素，具有良好的峰形。14个镧系元素的柱上分离，仅在7分钟内实现使用0.1 mmol/l铜铁试剂，15 mmol/l HIBA在pH 4.9。最佳分离条件下的分离效率在77000 ~ 208000理论板之间。 镧系元素配合物的测定通过在210 nm处直接UV检测来进行。检测限（信噪比=3）约为。镧系元素含量为0.24-0.47 μg/ml。在最佳条件下，实现了钍、铀与混合镧系元素的完全分离。
Separation and detection of lanthanides by capillary zone electrophoresis in the presence of cupferron (N-nitroso-N-phenylhydroxylamine) as UV absorbing complexing agent were investigated. The resolution of partially complexed positively charged cupferron complexes is improved by using a buffer ligand competing with cupferron for metal ions. When hydroxyisobutyric acid (HIBA) is used as buffer and competing ligand, it provides complete separation of all 14 lanthanides with good peak shapes. An on-column separation of 14 lanthanides was achieved in only 7 min using 0.1 mmol/l cupferron, 15 mmol/l HIBA at pH 4.9. The separation efficiencies for the optimum separation condition are between 77 000 and 208 000 theoretical plates. Determination of lanthanide complexes was performed by direct UV detection at 210 nm. Detection limits (signal-to-noise ratio=3) are ca. 0.24–0.47 μg/ml for lanthanides. Under optimum conditions, the complete separation of thorium and uranium from mixed lanthanides was achieved.