Retinoic acid disintegrated desmosomes and hemidesmosomes in stratified oral keratinocytes
Retinoic acid disintegrated desmosomes and hemidesmosomes in stratified oral keratinocytes
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DOI：
10.1111/j.1600-0714.2004.00245.x
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发表时间：
2004-11-01
影响因子：
3.3
通讯作者：
Satoh, M
中科院分区：
文献类型：
--
作者：
Hatakeyama, S;Hayashi, S;Satoh, M
背景：虽然已知视黄酸（RA）调节皮肤角质形成细胞的细胞分化，但RA对锚定连接的影响尚未阐明。探讨全反式RA对多层培养牙龈上皮(GE)1细胞的细胞-细胞和细胞-基质连接的影响。方法：观察GE1细胞的超微结构，并采用免疫组化法检测角蛋白4、角蛋白13和桥粒芯糖蛋白的表达。采用逆转录聚合酶链反应检测桥粒和半桥粒相关粘附分子、角蛋白 13 和角蛋白 14 的表达。结果：视黄酸导致角蛋白 4、角蛋白 13 和桥粒芯糖蛋白的免疫组织化学减少。从超微结构上看，RA 导致典型桥粒的急剧损失和半桥粒的完全损失。 RA 以剂量依赖性方式显着降低角蛋白 13、角蛋白 14、桥粒芯糖蛋白 1 和桥粒芯糖蛋白 1 的转录水平。 230-kD 大疱性类天疱疮抗原 (BPAG1) 基因表达也被 RA 降低，而整合素 α6、整合素 β4、180-kD 大疱性类天疱疮抗原 (BPAG2) 和层粘连蛋白 5 的转录水平不受影响。 结论：这些结果表明 RA 不仅通过剥夺细胞桥粒芯糖蛋白 1、桥粒胶蛋白来崩解桥粒。 1、角蛋白 13 和角蛋白 4，而且还通过减少基底角质形成细胞中 BPAG1 和角蛋白 14 的表达来影响半桥粒。
BACKGROUND: Although it is known that retinoic acid (RA) regulates the cellular differentiation of skin keratinocytes, the effects of RA on the anchoring junction have not been clarified. The effects of all-trans RA on cell-cell and cell-matrix connections of gingival epithelial (GE)1 cells in a multilayered culture were investigated.METHODS: Ultrastructures of GE1 cells were observed and immunohistochemistry was used to detect keratin 4, keratin 13, and desmoglein expression. Reverse transcription-polymerase chain reaction was performed to detect expression of desmosome and hemidesmosome-associating adhesion molecules, keratin 13, and keratin14.RESULTS: Retinoic acid caused immunohistochemical diminution of keratin 4, keratin 13, and desmoglein. Ultrastructurally, RA induced drastic loss of typical desmosomes and complete loss of hemidesmosomes. RA significantly decreased the transcript levels of keratin 13, keratin 14, desmoglein 1, and desmocollin 1 in a dose-dependent manner. The 230-kD bullous pemphigoid antigen (BPAG1) gene expression was also reduced by RA, whereas transcript levels of integrin alpha6, integrin beta4, the 180-kD bullous pemphigoid antigen (BPAG2), and laminin 5 were not affected.CONCLUSION: These results indicated that RA disintegrated not only desmosomes by depriving the cells of desmoglein 1, desmocollin 1, keratin 13, and keratin 4, but also hemidesmosomes by reducing the expression of BPAG1 and keratin 14 in basal keratinocytes.