Oxygen exposure effects on the dechlorinating activities of a trichloroethene-dechlorination microbial consortium
Oxygen exposure effects on the dechlorinating activities of a trichloroethene-dechlorination microbial consortium
复制标题
氧气暴露对三氯乙烯脱氯微生物群落脱氯活性的影响
DOI：
10.1016/j.biortech.2017.02.112
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发表时间：
2017-09-01
影响因子：
11.4
通讯作者：
Dong, Cheng-Di
中科院分区：
文献类型：
--
作者：
Liu, Na;Li, Haijun;Dong, Cheng-Di
这项工作的目的是研究氧气的存在对含有戴尔福特菌（D. mccartyi）的微生物群落脱氯三氯乙烯的影响。对功能细菌和非脱氯菌的16S rRNA以及还原脱氯基因进行了监测。将该群落暴露于氧气中改变了脱氯过程的整体生物转化速率，随着氧气浓度从0变为7.2毫克/升，生物转化过程延长，然而，三氯乙烯最终被脱氯为乙烯。定量聚合酶链反应（qPCR）分析表明，含有tceA基因的戴尔福特菌菌株比含有vcrA基因的戴尔福特菌菌株对氧气暴露的敏感性更低。通过Illumina MiSeq进行的高通量测序表明，非脱氯生物可能对清除氧气以保护戴尔福特菌免受损害至关重要。（C）2017爱思唯尔有限公司。保留所有权利。
The aim of this work was to study the effects of the presence of oxygen on the dechlorination of trichloroethene by a microbial consortium containing D. mccartyi. The 16S rRNA and reductive dechlorination genes of the functional bacteria and the non-dechlorinators were monitored. Exposing the consortium to oxygen altered the overall biotransformation rate of the dechlorination process, biotransformation processes prolonged with oxygen concentrations changing from 0 to 7.2 mg/L, however, trichloroethylene was eventually dechlorinated to ethene. The qPCR analyses revealed that the D. mccartyi strains containing the tceA gene were less sensitive to exposure to oxygen than were the D. mccartyi strains containing the vcrA gene. High-throughput sequencing by Illumina MiSeq indicated that the nondechlorinating organisms were probably crucial to scavenge the oxygen to protect D. mccartyi from being damaged. (C) 2017 Elsevier Ltd. All rights reserved.