Molecular evidence for ammonia oxidation bacteria in the sediments of shallow lake:A case study in Yangcheng Lake
Molecular evidence for ammonia oxidation bacteria in the sediments of shallow lake:A case study in Yangcheng Lake
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发表时间：
2012
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通讯作者：
Ruan Xiao-hong
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作者：
Ruan Xiao-hong
阳澄湖是长江三角洲的一个中型浅水湖泊，沉积物样品取自该湖。采用分子生物学方法对样品中的厌氧氨氧化菌和好氧氨氧化菌进行了检测。设计3对巢式PCR引物，对沉积物样品中的16 SrRNA进行扩增。利用引物AMX 368 f-AMX 820 r扩增出anammox特异性序列，并进行遗传学分析，结果表明，该序列与锦葵、锦葵和赤背锦葵的基因序列相似。amoA基因特异性引物从沉积物样品中扩增的序列属于β-变形菌纲。本研究为阳澄湖厌氧氨氧化与好氧氨氧化共存提供了分子证据。
Sediment samples were collected from Yangcheng Lake,a middle size shallow lake in Yangtze River Delta. Molecular biology methods were adopted to detect the anaerobic ammonia oxidation(anammox) and aerobic ammonia oxidation bacteria in the samples. Three pairs of nest PCR primers were designed to amplify the 16S rRNA from the sediment samples. The specified sequences of anammox were amplified by the primer pairs AMX368f-AMX820r,and analyses of phylogenetics showed that these sequences were similar to Candidatus brocadia fulgida,Candidatus brocadia anammoxidans and Candidatus scalindua. The sequences amplified by the amoA gene specified primer from the sediment samples belonged to the Betaproteobacteria class. This study provided the molecular evidence for coexistence of anammox and aerobic-ammonia-oxidation in Yangcheng Lake.