Poly-L-lysine-coated nanoparticles: A potent delivery system to enhance DNA vaccine efficacy
Poly-L-lysine-coated nanoparticles: A potent delivery system to enhance DNA vaccine efficacy
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DOI：
10.1016/j.vaccine.2006.09.086
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发表时间：
2007-01-26
期刊：
影响因子：
5.5
通讯作者：
Plebanski, Magdalena
中科院分区：
文献类型：
--
作者：
Minigo, Gabriela;Scholzen, Anja;Plebanski, Magdalena
DNA配方为安全和具有成本效益的疫苗提供了基础。然而，裸质粒DNA的免疫原性很差，需要新的有效的递送策略来提高DNA疫苗的效力。在这项研究中，我们提出了一种新的DNA疫苗递送方法，使用惰性聚l -赖氨酸（PLL）包被聚苯乙烯颗粒，这大大提高了DNA的免疫原性。皮内注射编码蛋清蛋白（OVA）的质粒DNA与pll包被的聚苯乙烯纳米颗粒复合物，可诱导C57BL/6小鼠体内产生高水平的CD8 T细胞和OVA特异性抗体，并进一步抑制表达EG7肿瘤细胞系的OVA攻击后的肿瘤生长。重要的是，疫苗的效力在很大程度上取决于所使用颗粒的大小以及PLL连接体的存在。我们的数据表明，pll包覆的聚苯乙烯纳米颗粒直径为0.05 μ m，而不是0.02 μ m或1.0 μ m，对于DNA疫苗的递送非常有效。(c) 2006 Elsevier Ltd.版权所有。
DNA formulations provide the basis for safe and cost efficient vaccines. However, naked plasmid DNA is only poorly immunogenic and new effective delivery strategies are needed to enhance the potency of DNA vaccines. In this study, we present a novel approach for the delivery of DNA vaccines using inert poly-L-lysine (PLL) coated polystyrene particles, which greatly enhance DNA immunogenicity. Intradermal injection of plasmid DNA encoding for chicken egg ovalbumin (OVA) complexed with PLL-coated polystyrene nanoparticles induced high levels of CD8 T cells as well as OVA-specific antibodies in C57BL/6 mice and furthermore inhibited tumour growth after challenge with the OVA expressing EG7 tumour cell line. Importantly, vaccine efficacy depended critically on the size of the particles used as well as on the presence of the PLL linker. Our data show that PLL-coated polystyrene nanoparticles of 0.05 mu m but not 0.02 mu m or 1.0 mu m diameter are highly effective for the delivery of DNA vaccines. (c) 2006 Elsevier Ltd. All rights reserved.