Discovery and genetic analysis of novel coronaviruses in least horseshoe bats in southwestern China.
Discovery and genetic analysis of novel coronaviruses in least horseshoe bats in southwestern China.
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DOI：
10.1038/emi.2016.140
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发表时间：
2017-03-29
影响因子：
13.2
通讯作者：
Liang G
中科院分区：
文献类型：
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作者：
Wang L;Fu S;Cao Y;Zhang H;Feng Y;Yang W;Nie K;Ma X;Liang G
为了调查蝙蝠冠状病毒（CoV），我们收集了132个直肠拭子和尿液样本，来自中国西南部的三个国家的五种蝙蝠。从最少的马蹄蝠样品中检测到7种冠状病毒，属于严重急性呼吸综合征（SARS）样冠状病毒和α-冠状病毒的不同群体。来自其他蝙蝠物种的样本对这些病毒呈阴性，表明最少的马蹄形蝙蝠代表了CoV菌株的天然储存库和混合器之一，并在这些病毒的进化和传播中发挥着关键作用。对这些菌株的遗传和进化特征进行了描述。对一个新分离株（F46）进行全基因组测序，结果表明，该分离株含有29699个核苷酸（不包括poly（A）尾）和13个开放阅读框（ORF）。F46基因的系统发育分析和重组分析表明，F46基因在蝙蝠SARS样冠状病毒（Rs3367和LYRa 11）和SARS冠状病毒（BJ 01）之间存在天然重组，表明F46可能是一种新的SARS样冠状病毒或SARS冠状病毒的重组病毒。
To investigate bat coronaviruses (CoVs), we collected 132 rectal swabs and urine samples from five bat species in three countries in southwestern China. Seven CoVs belonging to distinct groups of severe acute respiratory syndrome (SARS)-like CoVs and α-CoVs were detected in samples from least horseshoe bats. Samples from other bat species were negative for these viruses, indicating that the least horseshoe bat represents one of the natural reservoirs and mixers for strains of CoVs and has a pivotal role in the evolution and dissemination of these viruses. The genetic and evolutionary characteristics of these strains were described. Whole-genome sequencing of a new isolate (F46) from a rectal swab from a least horseshoe bat showed that it contained 29 699 nucleotides, excluding the poly (A) tail, with 13 open reading frames (ORFs). Phylogenetic and recombination analyses of F46 provided evidence of natural recombination between bat SARS-like CoVs (Rs3367 and LYRa11) or SARS-CoV (BJ01), suggesting that F46 could be a new recombinant virus from SARS-like CoVs or SARS-CoVs.