CREB-B acts as a key mediator of NPF/NO pathway involved in phase-related locomotor plasticity in locusts
CREB-B acts as a key mediator of NPF/NO pathway involved in phase-related locomotor plasticity in locusts
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CREB-B 是 NPF/NO 途径的关键介质，参与蝗虫相位相关的运动可塑性
DOI：
10.1371/journal.pgen.1008176
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发表时间：
2019-05-01
期刊：
影响因子：
4.5
通讯作者：
Wang, Xianhui
中科院分区：
文献类型：
--
作者：
Hou, Li;Li, Beibei;Wang, Xianhui
神经系统中基因表达的变化对于环境诱导的动物行为可塑性是必不可少的;然而，介导外部刺激对转录变化的影响的神经元信号通路在很大程度上是未知的。最近，我们已经证明，神经肽F（NPF）/一氧化氮（NO）信号通路发挥着调节作用的阶段相关的运动可塑性的飞蝗，飞蝗。在这里，我们报告，一个保守的转录因子，cAMP反应元件结合蛋白B（CREB-B），是一个关键的调解人参与的信号通路从NPF 2的NOS在飞蝗，触发运动活动之间的转换独居和群居阶段。我们发现CREB-B通过与NOS启动子区相互作用直接激活脑NOS表达。CREB-B的丝氨酸110位点的磷酸化随种群密度的变化而动态变化，并受NPF 2的负调控。RNA干扰实验和行为学实验进一步验证了CREB-B参与NPF 2调节的运动可塑性。此外，我们发现，蛋白激酶A介导的调节作用的NPF 2 CREB-B磷酸化和NOS转录。这些发现强调了环境诱导的行为可塑性背后的精确信号级联。
Gene expression changes in neural systems are essential for environment-induced behavioral plasticity in animals; however, neuronal signaling pathways mediating the effect of external stimuli on transcriptional changes are largely unknown. Recently, we have demonstrated that the neuropeptide F (NPF)/nitric oxide (NO) signaling pathway plays a regulatory role in phase-related locomotor plasticity in the migratory locust, Locusta migratoria. Here, we report that a conserved transcription factor, cAMP response element-binding protein B (CREB-B), is a key mediator involved in the signaling pathway from NPF2 to NOS in the migratory locust, triggering locomotor activity shift between solitarious and gregarious phases. We find that CREB-B directly activates brain NOS expression by interacting with NOS promoter region. The phosphorylation at serine 110 site of CREB-B dynamically changes in response to population density variation and is negatively controlled by NPF2. The involvement of CREB-B in NPF2-regulated locomotor plasticity is further validated by RNAi experiment and behavioral assay. Furthermore, we reveal that protein kinase A mediates the regulatory effects of NPF2 on CREB-B phosphorylation and NOS transcription. These findings highlight a precise signal cascade underlying environment-induced behavioral plasticity.