An architectural role of the Escherichia coli chromatin protein FIS in organising DNA
An architectural role of the Escherichia coli chromatin protein FIS in organising DNA
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DOI：
10.1093/nar/29.24.5107
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发表时间：
2001-12-15
影响因子：
14.9
通讯作者：
Muskhelishvili, G
中科院分区：
文献类型：
--
作者：
Schneider, R;Lurz, R;Muskhelishvili, G
大肠杆菌染色质蛋白FIS以生长阶段依赖的方式调节DNA的拓扑结构。在这项研究中，我们研究了FIS结合在体外对DNA结构的整体影响。我们发现，在超螺旋DNA分子中，FIS以非随机的方式结合在多个位置，并增加了DNA的分支。这种全球DNA重塑效应独立于FIS结合位点的螺旋相变。我们认为，除了先前推测的在某些启动子上游区域紧密弯曲的DNA微环的稳定外，FIS还可能执行组织大肠杆菌类核中的分枝Plectonemes的独特结构功能。
The Escherichia coli chromatin protein FIS modulates the topology of DNA in a growth phase-dependent manner. In this study we have investigated the global effect of FIS binding on DNA architecture in vitro. We show that in supercoiled DNA molecules FIS binds at multiple sites in a non-random fashion and increases DNA branching. This global DNA reshaping effect is independent of the helical phasing of FIS binding sites. We propose, in addition to the previously inferred stabilisation of tightly bent DNA microloops in the upstream regions of certain promoters, that FIS may perform the distinct architectural function of organising branched plectonemes in the E.coli nucleoid.