Do mycobacteria produce endospores?
Do mycobacteria produce endospores?
复制标题
分枝杆菌产生内生孢子吗？
DOI：
10.1073/pnas.0911299107
复制
发表时间：
2010
影响因子：
11.1
通讯作者：
Losick,Richard
中科院分区：
文献类型：
--
作者：
Traag,BjornA;Driks,Adam;Stragier,Patrick;Bitter,Wilbert;Broussard,Gregory;Hatfull,Graham;Chu,Frances;Adams,KristinN;Ramakrishnan,Lalita;Losick,Richard
The genusMycobacterium, which is a member of the high G+C group of Gram-positive bacteria, includes important pathogens, such asM. tuberculosisandM. leprae. A recent publication in PNAS reported thatM. marinumandM. bovisbacillus Calmette–Guérin produce a type of spore known as an endospore, which had been observed only in the low G+C group of Gram-positive bacteria. Evidence was presented that the spores were similar to endospores in ultrastructure, in heat resistance and in the presence of dipicolinic acid. Here, we report that the genomes ofMycobacteriumspecies and those of other high G+C Gram-positive bacteria lack orthologs of many, if not all, highly conserved genes diagnostic of endospore formation in the genomes of low G+C Gram-positive bacteria. We also failed to detect the presence of endospores by light microscopy or by testing for heat-resistant colony-forming units in aged cultures ofM. marinum. Finally, we failed to recover heat-resistant colony-forming units from frogs chronically infected withM. marinum. We conclude that it is unlikely thatMycobacteriumis capable of endospore formation.
