Identification of Mamu-DPA1, Mamu-DQA1, and Mamu-DRA alleles in a cohort of Chinese rhesus macaques
Identification of Mamu-DPA1, Mamu-DQA1, and Mamu-DRA alleles in a cohort of Chinese rhesus macaques
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DOI：
10.1007/s00251-013-0736-3
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发表时间：
2013-12-01
期刊：
影响因子：
3.2
通讯作者：
Wang, Xiaoning
中科院分区：
文献类型：
--
作者：
Deng, Qing;Zhang, Huiling;Wang, Xiaoning
恒河猴长期以来被用作各种人类疾病的动物模型；对其中一些疾病的易感性和/或抵抗力与主要组织相容性复合体（MHC）有关。为了深入了解MHC背景并促进中国恒河猴的实验使用，通过基因克隆和测序对30只中国恒河猴的Mamu-DPA1、Mamu-DQA1和Mamu-DRA等位基因进行了研究。本研究共鉴定出 14 个 Mamu-DPA1、17 个 Mamu-DQA1 和 9 个 Mamu-DRA 等位基因。在这些等位基因中，有 22 个新序列尚未在早期研究中记录，包括 9 个 Mamu-DPA1、10 个 Mamu-DQA1 和 3 个 Mamu-DRA 等位基因。有趣的是，与 Mafa-DQA1 和 Mafa-DPA1 一样，本研究在一只动物中检测到了两个以上的 Mamu-DQA1 和 Mamu-DPA1 等位基因，这表明它们可能代表基因重复。如果我们的研究结果能够得到其他研究的验证，将进一步增加已知的 Mamu-DPA1 和 Mamu-DQA1 多态性的数量。我们的数据还表明，中国恒河猴、越南食蟹猴和之前报道的恒河猴（主要来自印度）之间的 MHC II 类等位基因分布存在显着差异。这些信息不仅将促进对中华恒河猴MHC多样性和多态性的了解，也将有助于利用中华恒河猴进行人类疾病研究。
Rhesus macaques have long been used as animal models for various human diseases; the susceptibility and/or resistance to some of these diseases are related to the major histocompatibility complex (MHC). To gain insight into the MHC background and to facilitate the experimental use of Chinese rhesus macaques, Mamu-DPA1, Mamu-DQA1, and Mamu-DRA alleles were investigated in 30 Chinese rhesus macaques by gene cloning and sequencing. A total of 14 Mamu-DPA1, 17 Mamu-DQA1, and 9 Mamu-DRA alleles were identified in this study. Of these alleles, 22 novel sequences have not been documented in earlier studies, including nine Mamu-DPA1, ten Mamu-DQA1, and three Mamu-DRA alleles. Interestingly, like Mafa-DQA1 and Mafa-DPA1, more than two Mamu-DQA1 and Mamu-DPA1 alleles were detected in one animal in this study, which suggested that they might represent gene duplication. If our findings can be validated by other studies, it will further increase the number of known Mamu-DPA1 and Mamu-DQA1 polymorphisms. Our data also indicated significant differences in MHC class II allele distribution among the Chinese rhesus macaques, Vietnamese cynomolgus macaques, and the previously reported rhesus macaques, which were mostly of Indian origin. This information will not only promote the understanding of Chinese rhesus macaque MHC diversity and polymorphism but will also facilitate the use of Chinese rhesus macaques in studies of human disease.