Direct examination of the clonality of carcinogen-induced colonic epithelial dysplasia in chimeric mice.
Direct examination of the clonality of carcinogen-induced colonic epithelial dysplasia in chimeric mice.
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直接检查嵌合小鼠中致癌物诱导的结肠上皮发育不良的克隆性。
DOI：
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
M. M. Wilkinson
中科院分区：
文献类型：
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作者：
B. Ponder;M. M. Wilkinson
肿瘤病灶的克隆组成进行了检查，在组织切片的结肠上皮细胞的氧化偶氮甲烷（CAS号：25843-45-2）处理CBA/Ca-C57 BL/6 J小鼠聚集嵌合体，与H-2抗原作为细胞基因型的标志物。每55个早期肿瘤灶发生在马赛克补丁边界的细胞组成的单一基因型。我们的研究结果提供了直接的证据，这些病灶来自单一的隐窝。相比之下，17个较大的腺瘤的上皮细胞中有5个是混合基因型：在3个中，一种基因型仅由来自相邻隐窝的细胞学正常上皮细胞的边缘代表，但在其他2个中，两种基因型的上皮细胞发育不良。其中之一可能是一个“碰撞”肿瘤产生的相邻但独立的病灶，在其他少数组成部分可能是来自截留的非肿瘤性隐窝。
The clonal composition of neoplastic foci was examined in histological sections of the colonic epithelium of azoxymethane (CAS: 25843-45-2)-treated CBA/Ca----C57BL/6J mouse aggregation chimeras, with the use of H-2 antigens as markers of cellular genotype. Each of 55 early neoplastic foci occurring at a mosaic patch boundary was composed of cells of a single genotype. Our results provide direct evidence that these foci arise from single crypts. In contrast, the epithelium of 5 of 17 larger adenomas was of mixed genotype: In 3, one genotype was represented only by a rim of cytologically normal epithelium derived from adjacent crypts, but in the other 2 the epithelium of both genotypes was dysplastic. One of these was probably a "collision" tumor arising from adjacent but independent foci; in the other, the minority component may have been derived from entrapped non-neoplastic crypts.