Epidermal growth factor-induced stimulation of proliferation and gene expression changes in the hypotrichous ciliate, Stylonychia lemnae
Epidermal growth factor-induced stimulation of proliferation and gene expression changes in the hypotrichous ciliate, Stylonychia lemnae
复制标题
表皮生长因子诱导的下毛纤毛虫增殖刺激和基因表达变化
作者：
母伟杰;王琦;William A. Bourl;姜传奇;袁东霞;潘旭明;缪炜;陈瑛;熊杰
表皮生长因子(EGF)可诱导哺乳动物表皮和上皮组织的增殖。然而，EGF对单细胞真核生物，特别是原生生物的影响还没有得到很好的描述。纤毛虫是一类重要的原生动物，既是污染指标，又是研究的模式生物。柱头纤毛虫是最常见的自由生活纤毛虫之一，广泛分布于池塘、河流和沼泽中。在这里，我们报告了EGF在刺激Lemnae细胞增殖中的作用。生长曲线。建立狼疮模型，观察EGF对Fs增殖的刺激作用。Lemna进行了调查。根据这一结果，鉴定了潜在的EGF受体。Lemna根据保守结构域和基因表达。差异基因表达显示，EGF诱导的其他生物(如抗氧化剂)中的基因也上调INS。处于繁殖阶段的狐猴细胞。此外，我们的结果还表明，EGF可以上调OFS衰退期的信号转导相关过程。提示其具有抑制细胞凋亡的潜在功能。综上所述，本研究报道了首次研究EGF在纤毛虫中的作用，并建立了一个额外的系统来研究EGF在真核细胞分裂和增殖中的作用的分子机制。
Epidermal growth factor (EGF) induces proliferation of epidermal and epithelial tissues in mammals. However, the effect of EGF on the single-celled eukaryotes is not well characterized, especially in the protists. Ciliates, an important group of protists, are well characterized as both pollution indicators and model organisms for research.Stylonychialemnae, is one of the most common free-living ciliates, widely distributed in ponds, rivers and marshes. Here, we report the role of EGF on cell proliferation stimulation inS.lemnae. The growth curve ofS. lemnaewas established, and the stimulation effect of EGF on the proliferation ofS. lemnaewas investigated. Based on the results, potential EGF receptors were identified inS. lemnaeaccording to the conserved domains and gene expression. Differential gene expression revealed that EGF-induced genes in other organisms (e.g. antioxidant) also up-regulated inS. lemnaecells at propagation stages. In addition, our results showed that EGF could up-regulate the signal transduction-related processes in the decline stage ofS. lemnaecells, indicating its potential function in apoptosis inhibition. In summary, this study reports findings of the first investigation of EGF effects in hypotrich ciliates, and establishes an additional system for the study of the molecular mechanisms of EGF actions in eukaryotic cell division and proliferation.