OsTIR1 and OsAFB2 downregulation via OsmiR393 overexpression leads to more tillers, early flowering and less tolerance to salt and drought in rice.
OsTIR1 and OsAFB2 downregulation via OsmiR393 overexpression leads to more tillers, early flowering and less tolerance to salt and drought in rice.
复制标题
通过 OsmiR393 过表达下调 OsTIR1 和 OsAFB2 导致水稻分蘖增多、开花提前以及对盐和干旱的耐受性降低
DOI：
10.1371/journal.pone.0030039
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Zhang M
中科院分区：
文献类型：
--
作者：
Xia K;Wang R;Ou X;Fang Z;Tian C;Duan J;Wang Y;Zhang M
在拟南芥中，microRNA miR393通过靶向编码生长素受体的mrna，已被证明在植物发育和逆境反应中发挥作用。在本研究中，我们验证了OsmiR393 （Oryza sativa的Os）可以靶向两个水稻生长素受体基因同源物OsTIR1和OsAFB2。在osmir393过表达的水稻植株中观察到两种新的表型（分蘖增加和开花提前）和两种先前观察到的表型（耐盐性和耐旱性降低以及对生长素的敏感性降低）。osmir393过表达水稻对合成生长素类似物处理表现出低敏感性。这些数据表明，osmir393过表达水稻的表型可能是通过降低两种生长素受体基因（OsTIR1和OsAFB2）的表达而导致对生长素信号的敏感性降低。过量表达OsmiR393可下调生长素转运体OsAUX1和分蘖抑制剂OsTB1的表达，说明从OsmiR393到水稻分蘖的基因链可能是从OsTIR1和OsAFB2到OsAUX1，影响生长素的转运，再到OsTB1，最终控制分蘖。osmir393过表达水稻的阳性表型（分蘖增多、开花早）和阴性表型（耐盐性降低、生长素敏感性降低）给分子育种带来了困境。
The microRNA miR393 has been shown to play a role in plant development and in the stress response by targeting mRNAs that code for the auxin receptors in Arabidopsis. In this study, we verified that two rice auxin receptor gene homologs (OsTIR1 and OsAFB2) could be targeted by OsmiR393 (Os for Oryza sativa). Two new phenotypes (increased tillers and early flowering) and two previously observed phenotypes (reduced tolerance to salt and drought and hyposensitivity to auxin) were observed in the OsmiR393-overexpressing rice plants. The OsmiR393-overexpressing rice demonstrated hyposensitivity to synthetic auxin-analog treatments. These data indicated that the phenotypes of OsmiR393-overexpressing rice may be caused through hyposensitivity to the auxin signal by reduced expression of two auxin receptor genes (OsTIR1 and OsAFB2). The expression of an auxin transporter (OsAUX1) and a tillering inhibitor (OsTB1) were downregulated by overexpression of OsmiR393, which suggested that a gene chain from OsmiR393 to rice tillering may be from OsTIR1 and OsAFB2 to OsAUX1, which affected the transportation of auxin, then to OsTB1, which finally controlled tillering. The positive phenotypes (increased tillers and early flowering) and negative phenotypes (reduced tolerance to salt and hyposensitivity to auxin) of OsmiR393-overexpressing rice present a dilemma for molecular breeding.
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影响因子：
14.9
作者：
Chen C;Ridzon DA;Broomer AJ;Zhou Z;Lee DH;Nguyen JT;Barbisin M;Xu NL;Mahuvakar VR;Andersen MR;Lao KQ;Livak KJ;Guegler KJ
通讯作者：
Guegler KJ
DOI：
10.1186/1748-7188-3-3
发表时间：
2008-03-31
期刊：
Algorithms for molecular biology : AMB
影响因子：
--
作者：
Moxon S;Moulton V;Kim JT
通讯作者：
Kim JT
影响因子：
6.4
作者：
Sato, Atsuko;Yamamoto, Kotaro T.
通讯作者：
Yamamoto, Kotaro T.
影响因子：
3.7
作者：
Gleason C;Foley RC;Singh KB
通讯作者：
Singh KB
影响因子：
4.9
作者：
Endo-Higashi N;Izawa T
通讯作者：
Izawa T