Combining amphiphilic chitosan and bioglass for mediating cellular osteogenic growth peptide gene
Combining amphiphilic chitosan and bioglass for mediating cellular osteogenic growth peptide gene
复制标题
两亲性壳聚糖和生物玻璃组合介导细胞成骨生长肽基因
DOI：
10.1039/c5ra13628h
复制
发表时间：
2015-01-01
期刊：
影响因子：
3.9
通讯作者：
Chen, Xiaofeng
中科院分区：
文献类型：
--
作者：
Luo, Jie;Ling, You;Chen, Xiaofeng
基因治疗的实现很大程度上取决于有效的基因递送载体。生物活性玻璃（BG）作为骨诱导骨修复材料已广泛应用于临床。尽管有大量关于 BG 递送生物分子的研究，但迄今为止，很少有研究来调查 BG 作为聚合物/DNA 复合物增强剂的影响。在我们的研究中，我们使用合适大小的MBG来评估CS-PCL-mPEG/pOGP（含有成骨生长肽和绿色荧光蛋白融合基因）对不同类型细胞系中基因转染的影响。 MBG/CS-PCL-mPEG 更有效地浓缩和解离 pOGP。这些通过静电效应连接的复合物表现出较低且优化的 zeta 电位和低于 200 nm 的尺寸。我们的研究表明MBG具有良好的细胞生物相容性和高细胞摄取率。尽管两种细胞系之间存在差异，MBG/CS-PCL-mPEG 仍表现出增强的基因转染效率。这些结果表明 MBG 对聚合物基因载体的影响增强。因此，这一策略可能会拓宽生物玻璃在骨骼和牙齿修复和重建方面的生物医学应用。
The realization of gene therapy significantly depends on effective gene delivery vectors. Bioactive glasses (BG), as osteoinductive bone repair materials have been extensively applied clinically. Although there are numerous studies about the delivery of biomolecules by BG, to date, little study has been done to investigate the impact of BG as an enhancer for polymers/DNA complexes. In our study, we used an MBG with a suitable size to evaluate the influence of CS-PCL-mPEG/pOGP (containing the osteogenic growth peptide and green fluorescent protein fusion gene) on the gene transfection in different types of cell lines. MBG/CS-PCL-mPEG condense and dissociate the pOGP more effectively. These complexes, which are connected by the electrostatic effect, show lower and optimized zeta potential and size below 200 nm. Our study shows that MBG has good cellular biocompatibility and high cell uptake. MBG/CS-PCL-mPEG exhibits enhanced gene transfection efficiency despite the difference between the two cell lines. These results suggest that MBG has an enhanced impact on the polymer gene vectors. Therefore, this strategy may broaden the biomedical application of bioglass in the repair and reconstruction of bone and teeth.