A novel colorimetric aptasensor using gold nanoparticle for a highly sensitive and specific detection of oxytetracycline
A novel colorimetric aptasensor using gold nanoparticle for a highly sensitive and specific detection of oxytetracycline
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DOI：
10.1016/j.bios.2010.08.046
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发表时间：
2010-12-15
影响因子：
12.6
通讯作者：
Gu, Man Bock
中科院分区：
文献类型：
--
作者：
Kim, Yeon Seok;Kim, Joong Hyun;Gu, Man Bock
我们成功地开发了一种新型的比色适体传感器，使用纳米金颗粒来高灵敏和特异地检测土霉素(OTC)，土霉素是最常见的抗菌剂之一。利用与土霉素高亲和力结合的高度特异的单链DNA适配子来区分其他四环素类药物，如强力霉素(DOX)和四环素(TET)。由于核酸适体与靶分子的相互作用，OTC结合适配子(OBA)从金纳米粒子表面解吸，导致金纳米粒子的颜色从红色变为紫色，从而特异性地诱导了AuNPs的聚集。通过对适体用量的优化，OTC的检测下限提高到25 nM，比USA-EPA规定的下限低20倍，其线性动态范围为两个数量级。AuNPs和盐。该比色适体传感器具有信号产生简单、肉眼检测简单等优点，可实现抗菌剂的现场检测。(C)2010爱思唯尔B.V.保留所有权利。
We have successfully developed a novel colorimetric aptasensor using gold nanoparticles for highly sensitive and specific detection of oxytetracycline (OTC), one of the most common antibacterial agents. A highly specific ssDNA aptamer that bind to OTC with high affinity was employed to discriminate other tetracyclines (TCs), such as doxycycline (DOX) and tetracycline (TET). Aggregation of AuNPs was specifically induced by desorption of the OTC binding aptamers (OBAs) from the surface of gold nanoparticles as a result of the aptamer-target interaction, leading to the color change from red to purple. The detection limit of OTC was enhanced up to 25 nM, which is 20-fold lower than the limit USA-EPA regulated, with two orders of magnitudes in its linear dynamic range by successful optimization on the amount of the aptamers. AuNPs, and salts. This colorimetric aptasensor is advantageous over the other conventional methods in terms of its simple signal generation and detection with the naked eye, which can be realized in on-site detection of antibacterial agents. (C) 2010 Elsevier B.V. All rights reserved.