FUNCTIONAL DISSECTION OF DROSOPHILA-ABDOMINAL-B PROTEIN
FUNCTIONAL DISSECTION OF DROSOPHILA-ABDOMINAL-B PROTEIN
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DOI：
10.1016/0925-4773(91)90041-4
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发表时间：
1991-08-01
影响因子：
2.6
通讯作者：
BIENZ, M
中科院分区：
文献类型：
--
作者：
ALI, N;BIENZ, M
The Abdominal-B gene is unique among homeotic Drosophila genes as it encodes two proteins m and r which confer different functions. The m protein corresponds to an r protein with a large N-terminal extension; the two proteins contain the same homeodomain. We have used a transient co-transfection assay, based on HeLa cells, to analyse the intrinsic function of m and r protein in activating transcription. We find two strong transcriptional activation domains in the common part of the two proteins. The m-specific exon contains additional transcriptional activation potential. Despite this, the m protein is a weaker transcriptional activator than the r protein. Apparently, there are inhibitory sequences in the m-specific exon which, in the embryo, may have a role in masking r function in the intact m protein.
