Recombination can lead to spurious results in retroviral transduction with dually fluorescent reporter genes.
Recombination can lead to spurious results in retroviral transduction with dually fluorescent reporter genes.
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重组可能导致双荧光报告基因逆转录病毒转导产生虚假结果。
DOI：
10.1128/jvi.02524-13
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发表时间：
2013
影响因子：
5.4
通讯作者：
Johnson,MarcC
中科院分区：
文献类型：
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作者：
Salamango,DanielJ;Evans,DavidA;Baluyot,MarijuF;Furlong,JacksonN;Johnson,MarcC
荧光蛋白通常用作逆转录病毒载体中的报告基因。在这里，我们证明了转导与逆转录病毒载体携带串联二聚体番茄（TdTom）报告产生两个不同的荧光细胞群体模板跳跃由于第一和第二番茄基因之间的单核苷酸多态性。模板跳跃也发生在番茄和绿色荧光蛋白（GFP）基因的重复序列之间。因此，正确解释转导细胞的荧光强度需要谨慎。
Fluorescent proteins are routinely employed as reporters in retroviral vectors. Here, we demonstrate that transduction with retroviral vectors carrying a tandem-dimer Tomato (TdTom) reporter produces two distinct fluorescent cell populations following template jumping due to a single nucleotide polymorphism between the first and second Tomato genes. Template jumping also occurs between repeated sequences in the Tomato and green fluorescent protein (GFP) genes. Thus, proper interpretation of the fluorescence intensity of transduced cells requires caution.